نتایج جستجو برای: purified protein derivative
تعداد نتایج: 1328337 فیلتر نتایج به سال:
A serological technique, using formalinized red cells coupled to tuberculin PPD as antigen, has been assayed in a trial series of 124 tuberculous and 139 non-tuberculous patients. The stability of the antigen preparation, and the omission of an adsorption procedure for the removal of isoagglutinins facilitated the performance of the test. The results indicate that the technique warrants further...
The vaccine Bacillus of Calmette Guérin (BCG) was originally developed in France as an oral vaccine against tuberculosis. The oral use of this vaccine was replaced by the parenteral route in almost all countries after the Lubeck disaster. In contrast, Brazil retained the oral delivery of the vaccine until the mid-seventies when it was replaced by the intradermal route. This change in route of d...
Transformation of lymphocytes stimulated by mitogen, purified protein derivative, and in the mixed lymphocyte culture was significantly inhibited by gold sodium thiomate and gold sodium thiosulphate at concentrations of 5 X 10(-4) to 5 X 10(-5) mol/1.
Population control in dogs and cats is an important goal for many groups. Control measures over the years has included surgery, hormonal therapy and more recently immunological control. The current presentation discusses dog population control with an emphasis on immunologic control. Specifically, vaccination with purified zona pellucida (ZP) glycoproteins leads initially to immunocontraception...
In the various synthetic media proposed for growing Mycobacterium tuberculosis for tuberculin production, the emphasis has been on finding one which "would yield the greatest amount of growth per unit volume of culture medium in the shortest possible time" (Henley, 1929), on the tacit assumption that the amount of tuberculin produced parallels more or less directly such growth. Thus, Henley and...
A group of 1,974 cattle from Balcarce, Province of Buenos Aires (Argentina) was tuberculin tested in the neck with 0.1 mg of bovine PPD. In a second test carried out 35 days later, two doses of bovine PPDs from different laboratories and one dose of avian PPD were given to 90 of the cattle having shown a 5 mm or more increase in skin fold thick ness, and to 25 that were to be slaughtered for s...
Lymphadenitis is the most common manifestation of nontuberculous mycobacteria infection in children. Its frequency has increased over the past few decades. Diagnosis is based on clinical presentation, purified protein derivative skin test, and bacterial isolation. Management options are surgery, antibiotics, or "observation only"; however, the optimal therapy for this condition is still controv...
A microcytotoxic assay, based on the release of 51Cr from chicken erythrocytes by sensitized chicken lymphoid cells in the presence of the specific antigen, purified protein derivative (PPD), is described. The percentage of antigen-specific lysis was dependent on the lymphoid cell concentration, antigen concentration, time of incubation, and incubation temperature. Maximum antigen-specific lysi...
In this experiment 63 animals from a paratuberculosis (PTB) and tuberculosis-free herd were tested by Intradermal Tuberculin Tests (ITT) and blood samples were collected before PPD inoculation and on days 3, 15, 30, 60 and 90 post-inoculation (p.i.). Sera were tested for PTB-specific antibodies by ELISA-PPA and confirmed by a commercial ELISA. Three (4.76%) animals were positive by ELISA-PPA an...
OBJECTIVE To investigate whether the protective efficacy of bacille Calmette-Guérin (BCG) against tuberculosis decreases with time since vaccination. DESIGN A quantitative review of all 10 randomized trials of BCG against tuberculosis in purified protein derivative (PPD)-negative individuals, that presented data for discrete periods. For each trial, we derived log rate ratios for the annual c...
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