Ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) was developed to identify the absorbed parent components and metabolites in rat bile, plasma and urine after oral administration of Radix Paeoniae Alba extract (RPAE). A total of 65 compounds were detected in rat bile, plasma and urine samples, including 11 parent compounds an...

The identification of oxidation sites on a monoclonal antibody (mAb) using an Agilent 1260 Infinity HPLC-Chip/MS system coupled to an Agilent Accurate-Mass 6520 Quadrupole Time-of-Flight (Q-TOF) LC/MS is described. A monoclonal antibody was oxidized by treatment with various concentrations of t-butyl hydroperoxide (t-BHP) to stimulate potential oxidative modifications that may occur during a ma...

2,2-Diphenyl-1-picrylhydrazyl-ultra-high performance liquid chromatography-Q-time-of-flight mass spectrometry (DPPH-UPLC-Q-TOF/MS), as a rapid and efficient means, now was used for the first time to screen antioxidants from Selaginella doederleinii. The nine biflavone compounds were screened as potential antioxidants. The biflavones were structurally identified and divided into the three types,...

We attempted in this study to detect alginate oligosaccharides (AO) from mollusks. The samples used were digestive organs taken from turban shells and abalones which commonly ate brown algae. High-performance liquid chromatography (HPLC) and negative-ion electrospray ionization (ESI) quadrupole time-of-flight (Q-TOF) mass spectrometry (MS) analyses were used to confirm the presence of AO. Sampl...

The bioflavonoid quercetin occurs as a range of glycosides in foods, and the composition of these glycosides is species and cultivar specific. In humans, quercetin undergoes extensive phase II biotransformation, resulting in a complex mixture of metabolites. The bioactivity of the individual metabolites depends on the type and position of the conjugates formed during biotransformation. An Agile...

Evodia rutaecarpa (Juss.) Benth. (Rutaceae) dried ripe fruit is used for dispelling colds, soothing liver, and analgesia. Pharmacological research has proved that alkaloids are the main active ingredients of E. rutaecarpa. This study aimed to rapidly classify and identify the alkaloids constituents of E. rutaecarpa by using UPLC-Q-TOF-MS coupled with diagnostic fragments. Furthermore, the effec...

Xanthine oxidase (XO) plays an important role in purine degradation humans. The study aimed to determine the XO inhibitory potential of Chrysanthemum morifolium dried flower ethyl acetate sub-fractions and its anti-hyperuricemic effect rat models. Bioassay-guided fractionation based on assay was employed obtain bioactive fractions sub-fractions. In vitro cytotoxicity cellular antioxidant capaci...