AF8 cells were collected by mitotic detachment or made quiescent by serum restriction. Replated mitotic cells or serum-stimulated quiescent cells were then compared by flow cytofluorometry, with the use of acridine orange staining. Red fluorescence intensity (F ,„„,) was the same in quiescent cells and in cells immediately after mitosis. However, F „¿ „„ increased very rapidly in post...

Serum-deprived quiescent human diploid cells (HDC) were fused to replicative HDC, and DNA synthesis was monitored in the resulting heterodikaryons. Quiescent HDC had an inhibitory effect on DNA synthesis in replicative HDC nuclei in heterodikaryons. The timing of the inhibitory effect suggests that entry into S phase was inhibited but ongoing DNA synthesis was not inhibited in the replicative H...

introduction as a tumor grows, the demand for oxygen and nutrients increases and it grows further if acquires the ability to induce angiogenesis. in this study, we aimed to present a two-dimensional continuous mathematical model for avascular tumor growth, coupled with a discrete model of angiogenesis. materials and methods in the avascular growth model, tumor is considered as a single mass, wh...

Background: The significance of cTfh cells and their subsets in atherosclerosis is not well understood. We measured the frequency of cTfh subsets in patients with different degrees of stenosis using flow-cytometry. Methods: Participants included high (≥50%; n = 12) and low (<50%; n = 12) stenosis groups, as well as healthy controls (n = 6). Results: The frequency of CCR7loPD-1hiefficient-cTfh w...

The most detailed existing proposal for the structure of spacetime singularities originates in the work of Belinskii, Khalatnikov and Lifshitz. We show rigorously the correctness of this proposal in the case of analytic solutions of the Einstein equations coupled to a scalar field or stiff fluid. More specifically, we prove the existence of a family of spacetimes depending on the same number of...

Quiescence is a fundamental concept in modelling system behaviour, as it explicitly represents the fact that, in certain system states, no output is provided by the system. The notion of quiescence is also essential to model-based testing: if a particular implementation under test does not provide any output, then the test evaluation algorithm must decide whether to allow this behaviour, or not...