HpaI, a class II pyruvate-specific aldolase involved in the catabolic pathway of hydroxyphenylacetate, is overexpressed and purified. A previous suggestion that phosphate is involved in proton transfer of pyruvate, based on the crystal structure of the homologous 2-dehydro-3-deoxygalactarate aldolase, is not substantiated from biochemical studies with HpaI. Thus, specific activities of the enzy...

This paper is yet another solution for finding linear internal state assignments for the next-state behavior of a sequential machine. A state assignment is said to be linear if and only if the resulting next-state variables are linear combinations ofthe present-state and input variables. Consequently, the next-state behavior of a sequential machine is said to be linear if and only if there exis...

Background: Caenorhabditis elegans is an important model for the study of DNA damage and repair related processes such as aging, neurodegeneration, and carcinogenesis. However, DNA repair is poorly characterized in this organism. We adapted a quantitative polymerase chain reaction assay to characterize repair of DNA damage induced by ultraviolet type C (UVC) radiation in C. elegans, and then te...

determination of mobile localization with time of arrival (toa) signal is a requirement in cellular mobile communication. in some of the previous methods, localization with non-line-of-sight (nlos) paths can lead to large position error. also for simplicity, in most simulations suppose non stationary actual environments as stationary. this paper proposes (residual test + recursive least square)...