Preparations enriched in part-smooth (lacking ribosomes), part-rough (with ribosomes) transitional elements of the endoplasmic reticulum when incubated with ATP plus a cytosol fraction responded by the formation of blebbing profiles and approximately 60-nm vesicles. The 60-nm vesicles formed resembled closely transition vesicles in situ considered to function in the transfer of membrane materia...

Modified least squares method (LSM) is developed that take into account errors in input standard signals for calibration of measuring devices. Calibration of the differential pressure gage by the standard control-point setting mechanisms is demonstrated in the paper as an example. The accuracy of the coefficient estimates changes when the errors of the control-point setting mechanisms are taken...

Fucose 3H is a stable sugar selectively taken up into glycoproteins (1, 2) . Since fucose residues are located at the end of carbohydrate side chains (3), the uptake of fucose indicates completion of the synthesis of these side chains and presumably of the glycoprotein molecules themselves . When duodenal columnar cells were examined after fucose1H injection into rats, the label was observed on...

The intracellular migration of G protein in vesicular stomatitis virus-infected cells was visualized by light and electron microscope radioautography after a 2-min pulse with [3H]mannose followed by nonradioactive chase for various intervals. The radioactivity initially (at 5-10 min) appeared predominantly in the endoplasmic reticulum, and the [3H]mannose-labeled G protein produced was sensitiv...

A measurement apparatus is designed and fabricated to measure the effective thermal conductivity (keff) of a VIP (vacuum insulation panel) core specimen under various vacuum states and external loads. The apparatus consists of part for measuring keff, and parts for controlling external load and vacuum condition. Uncertainty of the apparatus is validated by measuring the standard reference mater...

A quantum measurement can be described by a set of matrices, one for each possible outcome, which represents the positive operator-valued measure (POVM) of the sensor. Efficient protocols of POVM extraction for arbitrary sensors are required. We present the first experimental POVM reconstruction that takes explicit advantage of a quantum resource, i.e., nonclassical correlations with an ancilla...

Surface tension is probably one of the most difficult phenomena to measure. Although a great deal of ingenuity has been spent for almost a century in devising accurate techniques, the figures obtained deviate more from each other for the same substance, according to different authors, than any other constant characterizing the substance. I t is well ,known tha t the two classes of methods of me...

The incorporation of [(3)H]fucose in the somatotrophic and gonadotrophic cells of the rat adenohypophysis has been studied by electron microscope autoradiography to determine the site of synthesis of glycoproteins and to follow the migration of newly synthesized glycoproteins. The pituitaries were fixed 5 min, 20 min, 1 h, and 4 h after the in vivo injection of [(3)H]fucose and autoradiographs ...