Materials Thrombin (bovine plasma, 1880 NIH units/mg protein) was purchased from Sigma (St. Louis, MO, U.S.A.). Simvastatin, fluvastatin and pravastatin were supplied by Merck & Co. (Rahway, N.J., U.S.A.), Novartis Pharma Inc. (Tokyo, Japan) and Sankyo Co. (Tokyo, Japan), respectively. TFLLR-NH2 (PAR1-activating peptide) and GYPGKFNH2 (PAR4-activating peptide) were purchased from Bachem (Bubend...

The spectra of the ferric form of most heme proteins [metmyoglobin, methemoglobin, horse radish peroxidase (EC 1.11.1.7), and ferricytochrome c at pH 1.5] are converted from high-spin (open crevice) structure to low-spin (closed crevice) form under pressure. Pressures up to 8000 kg/cm2 (780 MPa) have no effect on the spectra of high-spin ferro- and ferricytochrome c, which have a closed crevice...

Tungsten carbide nanorods (WC NRs) are demonstrated for the first time to possess intrinsic peroxidase-like activity towards typical peroxidase substrates, such as 3, 3', 5, 5'-tetramethylbenzidine (TMB) and ο-phenylenediamine (OPD) in the presence of hydrogen peroxide (H2O2). The reactions catalyzed by these nanorods follow the Michaelis-Menten kinetics. The excellent catalytic performance of ...

Effect of various concentrations of nickel (100, 200, 500 and 1000 microM) and recovery treatments of boron (50 and 100 microM) and copper (15 and 75 microM) each with 200 microM and 500 microM of nickel on germination, growth, biomass, chlorophyll, carotenoids, pheophytin, amylase, protein, sugar as well as activity of catalase and peroxidase were studied in radish (Raphanus sativus cv. Early ...

Alkyl esters of hydroxycinnamic acids and vanillin derivatives were used as model compounds in the oxidative coupling reactions catalyzed by a crude enzymatic extract Raphanus sativus var (red radish). Six products isolated characterized: three alkyl dimers vanilloids dimers. Herein, we report var. showed peroxidase activity catalyzing phenols. Vanilloid coupled an ortho-ortho way expected, whi...

Exposure of root tissue from a susceptible variety of sweet potato to low concentrations of ethylene induced a resistance to infection by Ceratocystis fimbriata and an increase in the activity of peroxidase and polyphenoloxidase in the tissue. Susceptible tissue that was inoculated with a pathogenic strain of C. fimbriata or a nonpathogenic strain that can induce resistance liberated more ethyl...