Nitrosomonas eutropha is an ammonia-oxidizing betaproteobacterium found in environments with high ammonium levels, such as wastewater treatment plants. The effects of NO(2) on gene and protein expression under oxic and anoxic conditions were determined by maintaining N. eutropha strain C91 in a chemostat fed with ammonium under oxic, oxic-plus-NO(2), and anoxic-plus-NO(2) culture conditions. Ce...

microbial cells are producers of natural polymers present in plant cells. production of pullulan (an extracellular microbial polysaccharide) by aureobasidium pullularia pullulans (p. pullulans) was studied under fermentation conditions, and kinetic parameters were determined. pullulan formation obeyed a growth and non-growth associated term. phb (polyhydroxybutyrates) an intracellular biopolyme...

Organic acids are considered as potential substrates for biosynthesis of polyhydroxyalkaonates. The acids may also be the metabolic inhibitors at moderate concentration levels. In this study, Ralstonia eutropha was used to elucidate the protein regulations when the bacterial cells pre-cultivated on glucose were exposed to three representative short chain organic acids, acetic, propionic and lev...

Maik Münzinger, Kambiz Taraz and Herbert Budzikiewicz* Institut für Organische Chemie der Universität zu Köln, Greinstr. 4, D-50939 Köln, Germany. Fax: + 4 9 -2 21 -470 -5057 . E-mail: [email protected] * Author for correspondence and reprint requests Z. Naturforsch. 54c, 867-875 (1999); received July 8, 1999 Ralstonia eutropha, Iron Transport, Siderophore, Staphyloferrin B The struct...

Class I polyhydroxybutyrate (PHB) synthase (PhaC) from Ralstonia eutropha catalyzes the formation of PHB from (R)-3-hydroxybutyryl-CoA, ultimately resulting in the formation of insoluble granules. Previous mechanistic studies of R. eutropha PhaC, purified from Escherichia coli (PhaC(Ec)), demonstrated that the polymer elongation rate is much faster than the initiation rate. In an effort to iden...

The enzymes chlorocatechol-1,2-dioxygenase, chloromuconate cycloisomerase, dienelactone hydrolase, and maleylacetate reductase allow Ralstonia eutropha JMP134(pJP4) to degrade chlorocatechols formed during growth in 2,4-dichlorophenoxyacetate or 3-chlorobenzoate (3-CB). There are two gene modules located in plasmid pJP4, tfdC(I)D(I)E(I)F(I) (module I) and tfdD(II)C(II)E(II)F(II) (module II), pu...