This study investigated the concentration/time dependent effects of nanosilver colloid on the motionparameters of the ejaculated ram spermatozoa in vitro. After incubation of sperm samples for 30, 60, 120 and 180 min in the presence of nanosilver colloid (0, 0.001, 0.01, 0.1, 1 and 10 ppm), the motion parameters were evaluated by computer assisted sperm analysis. There was a significant (P

In vitro induced apoptosis by dexamethazone treating of ram spermatozoa provoked the phospatidylserine exposure which is mainly localized on sperm tail region of plasma membrane and is correlated negatively with the sperm motility and survival rate.

Background: Egg yolk is the main cryoprotectant agent in semen freezing extenders which is used in order to protect spermatozoa against cold shock. However, elimination of animal bioproducts from the cryopreservation protocol is becoming mandatory. Therefore, the aim of this study is to compare a previously studied, homemade soya bean lecithin based extender with a commercially available extend...

Background: There are some methods for morphology staining of sperm. Although many different and also new methods are used in semen analysis, light microscopy is still used for routine morphological evaluation. The most used of the methods is Papanicolaou staining. This method is routinely used in Andrology laboratory for Human sperm morphology staining. In some domestic animals such as bull, R...

this study was conducted to evaluate the effect of egg yolk and cooling on ram coated spermatozoa. semen was collected from three ram by artificial vagina contacted with a tube containing tris- fructose-egg yolk 15%. samples were pooled, centrifuged by 700 g for 10 min and removed supernatant. then, samples were diluted by tris-glucose and centrifuged again to remove seminal plasma and egg yolk...

Antibiotics are used in extenders for sperm cryopreservation, however no specific regulation has been established for ram sperm. This study evaluates the effects of antibiotics in ram sperm extenders for bacterial control and its effect on sperm viability. Sperm from five rams was collected, cooled and frozen using extenders with the following antibiotics: 100,000 IU/mL penicillin and 100 μg/mL...

the aim of this study was to determine the effect of ewe breed on the reaction time and semencharacteristics in the ram. for this purpose, from the native sheep breeds in iran, seven lory rams and fourewes (two lory and two ghezel breeds) were selected. seven rams were individually subjected to 4 weeklytrials in which one of the following two treatments was applied alternately. all of rams were...

Re-suspension of frozen-thawed ram sperm with ovine and bovine whole seminal plasma (SP) is beneficial for its post-thawing viability. However, neither the influence of SP incubation duration nor the re-suspension with equine SP has been tested. In the first experiment, frozen-thawed ram sperm were incubated with SP from bovine (BSP), equine (ESP) or ovine (OSP) or without SP (-SP) for short (5...

The aim of this study was to determine the effect of ewe breed on the reaction time and semencharacteristics in the ram. For this purpose, from the native sheep breeds in Iran, seven Lory rams and fourewes (two Lory and two Ghezel breeds) were selected. Seven rams were individually subjected to 4 weeklytrials in which one of the following two treatments was applied alternately. All of rams were...