Numerous attempts have been made to control leptospirosis by using chemoprophylaxis, but with limited success. The present study was done investigate the antileptospiral potential of hexane, ethyl acetate and methanol extracts Zingiber zerumbet rhizomes. were assayed for activity broth microdilution method against Leptospira interrogans (serovar Batavie, Canicola, Australis) biflexa serovar Pat...

Accumulating evidence suggests that DNA-anti-DNA antibody immune complexes are endocytosed by cells, where they ligate intracellular nucleic acid sensors. This results in production of type I interferon or other cytokines, which may be a significant pathogenetic mechanism systemic lupus erythematosus (SLE). Most studies thus far have been performed using cell-lines peripheral blood mononuclear ...

The kinetics of formation and persistence of 7-ethylguanine (e7Gua) and O6-ethylguanine (O6eGua) were determined in rat liver and kidney DNA following i.p. injection with 12.5, 50, 100, or 200 mg DENA per kg body weight. The rate of ethylguanine formation in hepatic DNA was independent of carcinogen dose; however, the maximum level of DNA ethylation reached was linearly related to DENA dose. Pe...

F2408 rat cells transformed by polyoma virus contained integrated and nonintegrated viral DNA. The presence of nonintegrated viral DNA is under control of the A early viral function. Polyoma ts-a-transformed rat cells lose the free viral DNA when growth at the nonpermissive temperature (40 degrees C), but they reexpress it 1 to 3 days after they are shifted back to the permissive temperature. I...

A simple oncogene isolation was proved using the SD1-T rat embryonic cell line. The SD1-T cell line, which releases endogenous rat leukemia virus, was cocultured with (a) normal rat kidney cells transformed by cloned v-mos DNA, (b) the rat mammary tumor cell line (63SP), or (c) normal rat kidney cells transformed by 63SP DNA. Within 1 mo, oncogenic viruses were recovered from all three cocultur...

A lambda clone carrying a rat DNA fragment of 11.9 kb was isolated from a rat gene library with total rat tRNA as a probe. Nucleotide sequence analysis revealed that the DNA fragment contained six tRNA genes, three for tRNAPro and three for tRNALys. Of the six genes all but one tRNAPro gene have the same polarity. Each tRNA gene is separated by a DNA region of 0.1 to 3.6 kb. The 5'-flanking reg...

The purpose of this study was to compare the effects of rat colostrum and mature milk on newborn rat gastrointestinal growth under conditions that controlled the possible confounding effects of energy intake and mode of feeding. Newborn Sprague-Dawley rat pups were tubefed equicaloric amounts of rat colostrum or mature rat milk for 40 h before they were killed. Compared to littermates that were...

Rat embryos were explanted at 9.5 days and cultured for 48 h in human serum supplemented with glucose. The embryos were anaemic and frequently malformed. The haemoglobin and DNA content of these embryos was less than those grown in pure rat serum. Addition of 10% rat serum improved the embryonic growth as well as the haemoglobin and DNA content. This suggests that rat serum provides essential g...