in this study, decolorization of the diazo dye, reactive black 5 (rb5) in a coprinus cinereus peroxidase-catalyzed reaction has been investigated. a bisubstate kinetic model for the reaction rate based on the ping-pong mechanism was assumed for the enzymatic decolorization. experimentswere conducted at different rb5 and hydrogen peroxideconcentrations in a batch mannerto estimate the intrinsic ...

The present paper deals with the kinetics and mechanism of homogeneously Ir(III) chloride catalyzed oxidation of D-mannitol by chloramine-T [CAT] in perchloric acid medium in the temperature range of 30 to 45 0C. The reaction is carried out in the presence of mercuric acetate as a scavenger for chloride ion. The experimental results show first order kinetics with respect to the oxidant [CAT] an...

Dihydroorotase (DHO) is a zinc metalloenzyme that functions in the pathway for the biosynthesis of pyrimidine nucleotides by catalyzing the reversible interconversion of carbamoyl aspartate and dihydroorotate. A chemical mechanism was proposed on the basis of an analysis of the effects of pH, metal substitution, solvent isotope effects, mutant proteins, and alternative substrates on the enzyme-...

Upon treatment with carboxypeptidase, muscle aldolase (ketose-l-phosphate aldehyde-lyase, EC 4.1.2.7) loses 3 COOHterminal tyrosine residues (3, 4) and exhibits a 20-fold fall in the rate of fructose diphosphate cleavage (3) and a 500-fold fall in the rate of exchange detritiation of dihydroxyacetone phosphate (5). The cleavage of fructose-l-P is relatively unchanged (3). The present investigat...

Cryptochrome (CRY) is a blue-light sensitive flavoprotein that functions as the primary circadian photoreceptor in Drosophila melanogaster. The mechanism by which it transmits the light signal to the core clock circuitry is not known. We conducted in vitro studies on the light-induced conformational change in CRY and its effect on protein-protein interaction and performed in vivo analysis of th...