chlamydia trachomatis is one of the main causes of sexually transmitted diseases (stds) such as  prostatitis and epididymitis in men and cervicitis, endometriosis, vaginitis and ureogenital tract infections in women.  serological tests with sensitivities related to specific antigens are commonly used as  routine laboratory tests for diagnosis of chlamydia. in this research the chlamydia major o...

results cloning was confirmed by pcr and restriction digestion. rt-pcr and sds-page represented expression of kgf in e. coli. the optimized expression was achieved 16 hours after induction with 0.3 mm iptg at 37°c in luria broth (lb) containing kanamycin. the 18 kda protein was confirmed by western blotting, using anti-his antibodies. conclusions the result of the present study indicated that e...

conclusion our findings confirmed that a prokaryotic expression system of rureb229-561 was successfully constructed.the results of sds-page showed that our constructed prokaryotic expression system pet28a- ureb229-561 -bl21de3 efficiently produces target recombinant protein in the form of dissoluble inclusion body. therefore we can suggest that these epitopes can effectively be a vaccine candid...

background: although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. objective: the aim of this study was to clone the first 1100 bp of the c. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...

objective(s): hepatitis c is a major cause of liver failure worldwide. current therapies applied for this disease are not fully effective and produce side effects in most cases. non-structural protein 3 helicase (ns3) of hcv is one of the key enzymes in viral replication and infection. therefore, this region is a promising target to design new drugs and therapies against hcv infection. the aim ...

objective(s): enterotoxigenic escherichia coli (etec) strains are one of the primary causes of diarrhea in newborn calves and in humans, pigs, and sheep. igy technology has been identified as a promising alternative to generating a mass amount of specific antibody for use in immunotherapy and immunodiagnostics. the purpose of this study was to produce specific antibody by egg yolk antibody (igy...

citrus tristeza virus (ctv) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in iran, as one of the major citr...

Bovine ephemeral fever (BEF) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of Asia, Australia, and Africa. In recent years, BEF has been distributed in many provinces of Iran and caused economic losses. In this study the sequence encoding the G1 epitope of bovine ephemeral fever virus (BEFV) G glycoprotein was amplified by PCR, ligated into th...

The aim of the present study was to investigate the effects of recombinant Escherichia coli (E. coli) Trx-jingzhaotoxin (JZTX)-III on cell growth in the mouse hepatocellular carcinoma (HCC) cell line Hepa1-6. The JZTX-III gene sequence was synthesized and cloned into the pET-32a(+) vector to construct the recombinant fusion protein Trx-JZTX-III, which was subsequently purified. Hepa1-6 cells we...

  Objective(s): Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analy...