Bin-completion, a bin-oriented branch-and-bound approach, was recently shown to be promising for the bin packing problem. We propose several improvements to bin-completion that significantly improves search efficiency. We also show the generality of bin-completion for packing and covering problems involving multiple containers, and present bin-completion algorithms for the multiple knapsack, bi...

This paper briefl y presents two concepts of “RE” (1) re-empowering individuals using personal mobile technology reconstructed as measurement instruments and (2) re-cycle as a design constraint for extending a product’s usable lifetime. In the fi rst example we demonstrate the integration of simple low-cost, low-power air quality sensors attached to a mobile phone. In the re-cycle case, we demo...

M ultivesicular bodies (MVBs) are specialized endosomes that promote the degradation of membrane proteins by delivering them to lysosomes. This process is regulated by a series of endosomal sorting complexes required for transport (ESCRTs) (1). The ESCRT-0,-I, and-II complexes sequester ubiquitinated membrane proteins and recruit the ESCRT-III complex to drive the invagination and release of ca...

We have examined the effect of complete cell recycle on the production of cholera toxin (CT) by Vibrio cholerae and CT-like toxin by Vibrio mimicus in continuous culture fermentations. Complete cell recycle was obtained by filtering culture fluids through Amicon hollow fibers with an exclusion limit of 100,000 daltons (H1P100-20) and returning the concentrated cell slurry to the fermentor. A si...