Conditions of oxidative stress may lead to cataract formation. Reaction of certain flavoproteins, the NADH: oxidoreductases, with different quinones is well known to form hydrogen-peroxide. This reaction was investigated to get more information on cataract induction by naphthalene and its quinone metabolites. Protein extracts from bovine lens cortex exhibit "diaphorase" activity, indicated as d...

A commonly used enzymatic recycling assay for pyridine nucleotides has been adapted to directly measure the NAD(+)/NADH redox ratio in Drosophila melanogaster. This method is also suitable for quantification of NADP(+) and NADPH. The addition of a coupling reaction removing acetaldehyde produced from the alcohol dehydrogenase (ADH) reaction was shown to improve the linearity of NAD(H) assay. Th...

Intramolecular electron transfer within proteins is an essential process in bioenergetics. Redox cofactors are embedded in proteins, and this matrix strongly influences their redox potential. Several cofactors are usually found in these complexes, and they are structurally organized in a chain with distances between the electron donor and acceptor short enough to allow rapid electron tunneling....

The redox chemistry of CeIV-polyoxometalates towards proteins is linked to the redox-active residues on protein surface. It can be tuned by adjusting reaction parameters, directly impacting its efficiency and selectivity as an artificial protease.

Two dimensional silver(I) coordination polymer, [Ag(μ5-T4S)]n (1), (T4S- = toluene-4-sulfonate), has been synthesized and characterized by Inductively Coupled Plasma (ICP) and elemental analyses, IR spectroscopy and powder X-ray diffraction. This compound was calcined at 450, 500 and 700 °C in a furnace and static atmosphere of air. The resultin...

A theoretical model describing the transport and kinetic processes involved in heterogeneous redox catalysis of solution phase reactants at electrode surfaces coated with redox active monolayers is presented. Although the analysis presented has quite general applicability, a specific focus of the paper is concerned with the idea that redox active monolayers can be used to model an ensemble of i...

An alternative approach for numerical calculation of the square-wave voltammograms using the mathematical programming package MATHCAD is presented. A quasi-reversible redox reaction is considered and a mathematical model is developed under conditions of the square-wave voltammetry (SWV). Application of the mathematical model in MATHCAD is discussed and the file used for numerical simulation is ...

Redox processes are involved in almost every cell of the body as a consequence of aerobic life. In the past decades, redox biology has been increasingly recognized as one of the key themes in cell signaling. The progress has been accelerated by development of fluorescent probes that can monitor redox conditions and dynamics in cells and cell compartments. This short paper focuses on fluorescent...

The supported K2O–V2O5/Al2O3 catalytic system was designed to create surfaces that were 100% acidic, 100% basic, 100% redox, mixed redox-acidic and mixed redox-basic. The resulting nature of the surface sites was controlled by the impregnation of the specific additives (K-basic or V-redox/acidic), their order of impregnation and their surface coverage. The exact locations of the surface methoxy...

Proteins are capable of sensing the redox status of cells. Cysteine residues, which react with oxidants, reductants, and electrophiles, have been increasingly recognized as the mediators of this redox sensitivity. Cation channels encoded by the transient receptor potential (trp) gene superfamily are characterized by a wide variety of activation triggers that act from outside and inside the cell...