A permanently high virulence was found in tachyzoites of T. gondii K24 after serial passage in mice (90 passages during 324 days). Virulence tests revealed that a single tachyzoite of the 50th passage represented LD100 for mice. Analysis of genotype of K24 isolate was done by PCR/RFLP with ROP1/Ddel, SAG1/Ddel, 850/Rsal and IGS/Rsal and by RFLP/DNA with TGR1E sequence and Pstl enzyme. K24 isola...

PCR-restriction fragment length polymorphism (RFLP) analysis of a 960-bp fragment of the Campylobacter gyrB gene with either DdeI or XspI restriction enzymes generated unique digestion patterns for 12 different Campylobacter species. In addition, PCR assays using species-specific primer sets targeting gyrB were specific for the respective Campylobacter species. Therefore, PCR-RFLP analysis and ...

background chronic hepatitis b virus (hbv) infection is a multifactorial disease that can result in serious clinical complications. host genetic background especially the genes that encode immunologic factors like inf-γ and its receptor (ifn-γ r) are critical in the pathogenesis of infection. materials and methods genomic dna from peripheral blood samples of 200 chronically hbv infected patient...

background: aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, na­sal sinusitis and invasive infection. in this study, we developed a pcr-single strand confomational polymorphism method to identify the most common aspergillus species and we showed some advantages of this method comparing a pcr-restric­tion fragment length polymorphism with ...

an attempt has been made to analyze the distribution of the beta-lactoglobulin genotype in swamp buffaloes and murrah buffaloes utilizing polymerase chain reaction-restriction fragment length polymorphism(pcr-rflp). blood samples were taken from 50 swamp buffaloes and 50 murrah buffaloes. the dna was extracted by the phenol-chloroform method. the pcr-rflp was performed using beta-lactoglobulin ...

results we identified no mutations in these patients in the three screened positions. conclusions to ensure that these genes play no role in the disease, evaluation of the non-coding regions of both the cyp1b1 and myoc genes is strongly recommended, since other genes are involved in the pathogenesis of glaucoma. patients and methods forty patients with poag were recruited from the ophthalmic di...

a polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) test was performed to investigate the allele frequencies of the insulin-like growth factor (igf1 and igf2 genes) in 90 broilerchickens. a 793 bp fragment of igf1 gene and 1146 bp fragment of igf2 gene were amplified and digested with hinfi and hsp92ii restriction enzymes,respectively. two types of alleles of a and b...

ThirtyFrieswal lactating cows were screened for clinical and subclinical mastitis and subsequently classified into healthy, subclinically affected and clinically affected groups each group comprising of 10 cows. Polymorphism of cow lactoferrin (LTF) gene promoter was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that LFT gene pro...

several asthma susceptibility loci, including a region containing the vitamin d receptor (vdr) gene located at chromosome 12q, have been identified using genome-wide screens. our aim is to investigate the association between single nucleotide polymorphisms (snps) in vdr gene and asthma. one hundred one asthma patients and 206 healthy controls were enrolled in this study. genotypes were determin...

A total of 121 reference and clinical strains of both slowly and rapidly growing mycobacteria belonging to 54 species were studied for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock protein. Restriction digests were separated by 10% polyacrylamide gel electrophoresis (PAGE). By including a size standard in each sample, the r...