Male infertility is a reproductive disease caused by various factors, including environmental factors and genetic defects. Thousands of genes have been identified to cause associate with male infertility, such as TEX15. Our study aimed identify the association between polymorphism TEX15 rs142485241 infertility. Total DNAs were extracted from whole blood 429 unrelated Vietnamese individuals, 202...

Using the PCR-RFLP method, we genotyped pigs for genes GBP1 (mutation c. [10A>G; 11A>G]), FUT1 G307A) and MUC4(A243G) associated with resistance to infectious diseases. A total of 188 boars four breeds (Duroc, Large White, Landrace Yorkshire) from Belgorod Region Russian Federation were studied. The highest frequency favorable genotype AG polymorphism GBP1E2, which is resistant pig respir...

Huanglongbing (yellow dragon disease) is a destructive disease of citrus. The etiological agent is a noncultured, phloem-restricted alpha-proteobacterium, "Candidatus Liberibacter africanus" in Africa and "Candidatus Liberibacter asiaticus" in Asia. In this study, we used an omp-based PCR-restriction fragment length polymorphism (RFLP) approach to analyze the genetic variability of "Ca. Liberib...

Objective
 Type 2 diabetes mellitus (T2DM) and diabetic
 retinopathy (DR) development is affected by genetic
 factors. Matrix metalloproteinases (MMPs) are involved
 in many cellular processes, such as remodeling of
 extracellular matrix proteins angiogenesis. In
 this study, we investigated the relationship between
 MMP9 −1562 C/T (rs3918242) polymorphism the...

This study was carried out on 114 Brown Swiss cattle reared in intensive conditions at Ataturk University Food and Livestock Application Research Center the private farm found Erzurum province. Genotypic structures were examined terms of Insulin-like growth factor (IGF)-II gene locus distribution genotypes allele frequencies concerning genes determined. The identified associated with milk yield...

A new method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of a point mutation of the Bruton’s tyrosine kinase (Btk) gene in CBA/N mice bearing an X-linked recessive immunodeficiency (xid). Since restriction site useful for RFLP analysis does not exist in the spontaneous mutant Btk locus, an artificial restriction site...

The availability of a variety of restriction endonuclease enzymes that cleave deoxyribonucleic acid (DNA) at specific sites has made it possible to identify the presence of polymorphic regions in the isolated fragments. Such restriction fragment length polymorphism (RFLP) results owing to a variation in the number of tandem repeats (VNTR) of a short DNA segment. These VNTR sequences can uniquel...

The combination of preventive vaccination and diagnostic typing of viral isolates from patients with clinical poliomyelitis constitutes our main protective shield against polioviruses. The restriction fragment length polymorphism (RFLP) adaptation of the reverse transcriptase (RT)-PCR methodology has advanced diagnostic genotyping of polioviruses, although further improvements are definitely ne...

Background: The worldwide distribution of P. vivax has expanded significantly and the number of reported cases has been on the rise. Approximately 88% of malaria cases in Iran are caused by Plasmodium vivax, and in order to management of the disease, understanding the population genetic structure of the parasite is necessary for designing and applying drugs and vaccines. Among many potential ca...

Insertion-deletion polymorphism (InDeL) is the second most frequent type of genetic variation in the human genome. For the detection of large InDeLs, researchers usually resort to either PCR gel analysis or RFLP, but these are time consuming and dependent on human interpretation. Therefore, a more efficient method for genotyping this kind of genetic variation is needed. In this report, we descr...