A major problem in the formulation of therapeutic proteins is the irreversible protein aggregation. Recombinant human interferon alpha2b (rhIFN2b) has poor stability and undergoes physical degradation. The aim of this study was to investigate the effect of solution conditions on the heat-induced aggregation of rhIFNα2b. The protein was incubated for 1 h at 40°C–70°C and for up to 240 h at 50C...

Neutrophils play a key role in the pathophysiology of septic multiple organ dysfunction syndrome (MODS) through excessive release of toxic granule components and reactive oxygen metabolites with consequent tissue destruction. The increase of senescent neutrophils during sepsis indicates a potential breakdown of autoregulatory mechanisms including apoptotic processes to remove activated neutroph...

Mendelian susceptibility to mycobacterial diseases (MSMD) is a rare inheritance syndrome, characterized by a disseminated infection with mycobacterium in children following BCG vaccination at birth. Regarding the vaccination program in Iran, it may consider as a public health problem. The pathogenesis of MSMD is dependent on either insufficient production of IFN-gamma (γ) or inadequate response...

mendelian susceptibility to mycobacterial diseases (msmd) is a rare inheritance syndrome, characterized by a disseminated infection with mycobacterium in children following bcg vaccination at birth. regarding the vaccination program in iran, it may consider as a public health problem. the pathogenesis of msmd is dependent on either insufficient production of ifn-gamma (γ) or inadequate response...

Interleukin-12 (IL-12) and interferon-gamma (IFN-gamma) exert protective effects during experimental endotoxemia through upregulation of cellular immunity and phagocytic functions. They are part of a positive regulatory feedback loop that enhances the production of the other. Because critically ill patients show a marked suppression of T-cell and macrophage functions with a high susceptibility ...

130 protein-based therapeutics are licensed today. With the addition of anticipated new biotherapeutics, the market is forecasted to exceed $140 billion USD in 2017. These therapeutic drugs have changed and improved the quality of life of millions of people around the world in the past few decades. One of the most important quality attributes of a biopharmaceutical drug is its heterogeneity, wh...

Recombinant human interferon(rhIFN) is the leading therapeutic intervention shown to change the cause of relapsingremitting multiple sclerosis, and both a nonglycosylated and a significantly more active glycosylated variant of rhIFNare used in treatment. This study investigates the function of the rhIFN1a glycan moiety and its individual carbohydrate residues, using the myxovirus resistance (Mx...