Mutualistic interactions with fungal endophytes and dinitrogen-fixing bacteria are known to exert key biological influences on the host plant. The influence of a fungal endophyte alkaloid on the toxicity of a plant has been documented in Oxytropis sericea. Oxytropis sericea is a perennial legume responsible for livestock poisoning in western North America. Livestock poisoning is attributed to t...

Somatic antigens of Bradyrhizobium japonicum, Rhizobium sp. (Cicer arietinum) and Rhizobium sp. (Leucaena leucocephala) were prepared as standard, single-species type from cultured cells. Equal numbers of the cells of these rhizobia were then combined to obtain a mixed-rhizobial-species antigen preparation. Rabbits were immunized either with the standard, single-species type or with the mixed-r...

A symbiotic strain was isolated from the root nodules of the legume pulse Phaseolus mungo L and designated as PM 25. The strain was identified as Rhizobium sp. on the basis of the physiobiochemical characteristics and 16S rDNA sequence based homology. The Minimum Inhibitory Concentration of the analogue 5-fluro-tryptophan was 3.0 mM. The analogue (5FT) resistant mutants were screened from wild ...

The complete nucleotide sequence of the nodS gene from the bean-nodulating Rhizobium etli, presumably encoding a methyltransferase, was determined. A phylogenetic analysis of five different NodS proteins from three genera of Gram- soil bacteria, Azorhizobium, Bradyrhizobium and Rhizobium, was performed.

Rhizosphere engineering with beneficial plant growth promoting bacteria offers great promise for sustainable crop yield. Potato is an important food commodity that needs large inputs of nitrogen and phosphorus fertilizers. To overcome high fertilizer demand (especially nitrogen), five bacteria, i.e., Azospirillum sp. TN10, Agrobacterium sp. TN14, Pseudomonas sp. TN36, Enterobacter sp. TN38 and ...

Dot immunablot assay was used for determination of nodules produced on soybean. french bean. pigeon pea and urdbean by Bradyrhizobiumjaponicum USDA-I 10; R. leguminosarum bv. phaseoli FB-77 and N-3; Rhizobium sp. A-3 and U-I. respectively. Nodule occupancy by inoculated strains as determined by the test ranged between 73 to 93%. Replica inmrunoblot assay reduced the time required for enumeratio...

A Gram-negative, non-motile, pale-yellow, rod-shaped bacterial strain, DS-42(T), was isolated from a soil in Korea and its taxonomic position was investigated by a polyphasic study. Strain DS-42(T) grew optimally at 25 degrees C and pH 7.0-8.0. Strain DS-42(T) did not form nodules on three different legumes, and the nodD and nifH genes were also not detected by PCR. Strain DS-42(T) contained Q-...

Rhizobia are a group of bacteria that form nodules on the roots of legume host plants. The sequenced genomes of the rhizobia are characterized by the presence of many putative insertion sequences (IS) elements. However, it is unknown whether these IS elements are functional and it is therefore relevant to assess their transposition activity. In this work, several functional insertion sequences ...

A new Rhizobium species that nodulates Phaseolus vulgaris L. and Leucaena spp. is proposed on the basis of the results of multilocus enzyme electrophoresis, DNA-DNA hybridization, an analysis of ribosomal DNA organization, a sequence analysis of 16S rDNA, and an analysis of phenotypic characteristics. This taxon, Rhizobium tropici sp. nov., was previously named Rhizobium leguminosarum biovar ph...