Two typing methods were evaluated, utilizing 62 clinical strains of Pseudomonas aeruginosa, to assess their usefulness as tools to study the bacterial diversity within this complex group. Genetic diversity was determined by PCR ribotyping and enterobacterial repetitive intergenic consensus (ERIC) PCR. By these methods, 9 and 36 genotypes were found, respectively. The result showed that ERIC PCR...

Salmonella enterica serovar Typhi strains resistant to ampicillin, chloramphenicol, tetracyclines, streptomycin, and cotrimoxazole, isolated from sporadic cases and minor outbreaks in Vietnam between 1995 and 2002, were typed and compared. Plasmid fingerprinting, Vi bacteriophage typing, XbaI pulsed-field gel electrophoresis, and PstI ribotyping showed that endemic, epidemic multidrug-resistant...

background and objective: over 165 million cases of shigellosis occur in the world each year, mostly in developing countries. outbreaks of shigellosis are associated with poor sanitation, natural calamities, contaminated food and crowded living conditions. in late summer 2006, during the final stage of an outbreak of shigellosis at a vast region of isfahan province, naein & ardestan, our labora...

Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grou...

We studied the restriction fragment length polymorphism of the rRNA gene and CTX genetic element in Vibrio cholerae O139 Bengal, which resurged in Calcutta in September 1996 after a gap of 32 months. While the strains from this resurgence were indistinguishable from the earlier strains by ribotyping, the structure of the CTX genetic element present in the current O139 strains was found to be un...

Ribotyping, a molecular method based on the analysis of restriction fragment length polymorphisms (RFLPs) of ribosomal RNA genes, has been widely adopted to distinguish a variety of bacteria (1-5). For the past five years ATCC has been using the RiboPrinter® Microbial Characterization system (Qualicon Inc., Wilmington, DE) as a primary method for characterizing strains during the quality contro...

background and objectives: pseudomonas aeruginosa possesses a variety of virulence factors that may contribute to its pathogenicity. the aim of this study was to evaluate opri , oprl and toxa genes for pcr identification of clinical p. aeruginosa . in order to find out any relation between special virulence factors and special manifestation of p. aeruginosa infections, we detected virulence fac...