An indirect immunofluorescence technique was employed to determine the intracellular distribution of the regulatory subunit of type II cyclic adenosine 3':5'-monophosphate-dependent protein kinase (RII) in Y-79 human retinoblastoma cells growing in monolayer culture. During the initial phases of growth (6 hr-6 days after seeding), RII was confined to the cytoplasmic areas of the Y-79 cells, see...

Pembangunan Pembangkit Listrik di Indonesia pada program Fast Track Program 1 (FTP-1) 2006-2009 mengalami keterlambatan 5 Tahun dan hanya sekitar 74% yang terselesaikan. Keterlambatan pelaksanaan proyek berbanding lurus dengan Cost Overrun. M-PERT sangat efektif dalam optimasi proyek, hampir mencapai 99%. Penelitian ini menggunakan kombinasi penelitian statistik divalidasi terhadap studi kasus....

8-Cl-cAMP, a site-selective cAMP analog, induces growth inhibition in a variety of cell types of human cancer cell lines. This inhibitory effect of 8-C1-CAMP was related to its ability to differentially regulate type I versus type I1 CAMP-dependent protein kinase. In the present study we demonstrated a unique mechanism of action of 8-Cl-CAMP in the regulation of these kinase isozymes in HL-60 h...

Siddiqui, Shahid S., Zeba K. Siddiqui, and Asrar B. Malik. Albumin endocytosis in endothelial cells induces TGFreceptor II signaling. Am J Physiol Lung Cell Mol Physiol 286: L1016–L1026, 2004. First published January 16, 2004; 10.1152/ajplung.00356.2003.—Vascular endothelial cells undergo albumin endocytosis using a set of albumin binding proteins. This process is important for maintaining cell...

site for AKAPs the PKA an “hydrophobic Although a p dimers contai chains, the co generates a re difference in t posed as a me differentially i Highly loca by the uniqu associated wit AKAPs for RII variations wit late with diffe site (2, 111). F called Ht31, domain of AK to disrupt RII: much higher 1,030–1,277 n affinity betw AKAPs have a tion can be m analysis, and demonstrated affinities for s...

Simple Landau free energy function is presented to describe the Liquid-RII-RI phase sequence of alkanes and transitions between them. The order parameters necessary to describe these rotator phase transitions are identified. We present a mean-field description of the Liquid-RII and Liquid-RI transitions. General arguments are presented for the topology of the phase diagram in the vicinity of th...

OBJECTIVE To determine whether a specific component of hostility (i.e. cognitive or behavioural) may predict suicide in a prospective design, controlling for depressive mood. METHOD In 1993, 14,752 members of the 'GAZ et ELectricité' (GAZEL) cohort (10,819 men, mean age=49.0 years; 3933 women, mean age=46.2 years) completed the Center for Epidemiologic Studies Depression Scale and at least on...

BACKGROUND Proinflammatory cytokines are important mediators in heart failure (HF). Recently, urinary levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) have been determined. AIMS The purpose of this study was to measure the urinary levels of TNF-alpha and IL-6 receptors, sTNF-RI, sTNF-RII, sIL-6R, and the relationship with plasma levels and NYHA classes in HF. METHO...

The aim of this study was to evaluate the structure and measurement invariance religious identity, practice, beliefs across cultures in six world regions (Asia, non-Western Europe, North America, Oceania, South Western Europe) Western, Educated, Industrialized, Rich, Democratic (WEIRD) non-WEIRD regions. Confirmatory factory analysis examined whether hypothesized model fits data; several multi-...

A method is described for the separate determination of cAMP intracellularly bound to the regulatory moieties (RI and RII) of protein kinase I and II. The cAMP endogenously bound to RI or RII in hepatocyte extract was adsorbed to protein A-agarose beads coated with antibodies against RI or RII. The endogenously bound cAMP was eluted from the washed beads with dilute acetic acid before being ass...