OBJECTIVE The human methylenetetrahydrofolate reductase (MTHFR) gene plays a crucial role in folate metabolism. Data regarding the influence of MTHFR gene polymorphisms on male fertility status are scarce and conflicting. We determined associations between 3 MTHFR gene polymorphisms (C677T, A1298C, and G1793A), serum folate, and total homocysteine (tHcy) levels, with male fertility status and s...

background: the aim of this study was dna extraction from protosco-lecses of echinococcus granulosus and identification of these strains in west- azerbaijan province, north western iran. methods: thirty one livestock isolates from sheep and cattle were collected from abattoirs of the province. to investigate the genetic variation of the isolates, after dna extraction by glass beads-phenol chlor...

BACKGROUND A longitudinal Anopheles gambiae s.l. insecticide resistance monitoring programme was established in four sentinel sites in Chad 2008-2010. When this programme ended, only sporadic bioassays were performed in a small number of sites. METHODS WHO diagnostic dose assays were used to measure the prevalence of insecticide resistance to 0.1% bendiocarb, 4% DDT, 0.05% deltamethrin, 1% fe...

OBJECTIVE To determine comparative fracture risk in HIV patients compared with uninfected controls. DESIGN A randomised cross-sectional study assessing bone mineral density (BMD), fracture history and risk factors in the 2 groups. SETTING Hospital Outpatients. SUBBBJECTS 222 HIV infected patients and an equal number of age-matched controls. ASSESSMENTS Fracture risk factors were assesse...

Parkinson's disease (PD) is caused by selective dopaminergic cell loss in the nigro stratal system. The etiology of PD is a combination of genetic and environmental factors. The polymorphism of the detoxification enzyme NAT2 is responsible for the individual differences in the acetylating rate of aromatic amine and hydrazine-drugs, chemical and environmental pollutants (potential carcinogens an...

PURPOSE To study whether -160 C > A (rs16260) and -347 G > GA (rs5030625) single nucleotide polymorphisms of the regulatory region (rSNPs) of CDH1 gene modulate the risk of nephrolithiasis. METHODS Genomic DNA of 101 patients with calcium oxalate nephrolithiasis and 114 healthy controls were screened for both polymorphisms, using polymerase chain reaction-restriction fragments length polymorp...

INTRODUCTION Ten viral genotypes (A-J) distributed in all continents have been described for hepatitis B virus (HBV). One of the methodologies for determining the viral genotype is the restriction fragment length polymorphism (RFLP) technique, a simple and relatively inexpensive method, albeit with some limitations. OBJECTIVE The initial objective of the project was to identify the HBV genoty...

26 27 Objective: The peroxisome proliferator-activated receptor (PPAR)gamma is a 28 transcription factor involved in glucose homeostasis and energy metabolism. 29 A missense mutation at codon 12 in the PPARgamma2 has been associated 30 with increased body mass index and attenuated Insulin Resistance (IR) in 31 PCOS. We have recently shown a decreased Basic Metabolic Rate (BMR) in 32 PCOS. The a...

The purpose of the present study was to compare the sensitivity and specificity of V3 enzyme immunoassay (solid phase EIA and EIA inhibition) and restriction fragment length polymorphism (RFLP) with the DNA sequencing "gold standard" to identify the Brazilian HIV-1 variants of subtype B and B"-GWGR. Peripheral blood mononuclear cells were collected from 61 HIV-1-infected individuals attending a...

The aim of the paper is to determine whether IGF1, IGFBP3 and IGF1/IGFBP3 molar ratio in addition to PSA and one-nucleotide polymorphism in PSA and CYP17 gene might contribute to early diagnostics of prostate cancer (PCa). Serum level of PSA, IGF1 and IGFBP3 in the group of 158 individuals (92 PCa and 66 controls) was examined by RIA method and IGF1/IGFBP3 was calculated. PCR RLFP method was us...