Late bolting after cold exposure is an economically important characteristic of radish (Raphanus sativus L.), an important Brassicaceae root vegetable crop. However, little information is available regarding the genes and pathways that govern flowering time in this species. We performed high-throughput RNA sequencing analysis to elucidate the molecular mechanisms that determine the differences ...

BACKGROUND AND AIMS Bolting, the first visible sign of reproductive transition in beets (Beta vulgaris), is controlled by the dominant bolting gene B (B allele), which allows for flowering under long days (LDs, >14 h light) without prior vernalization. The B-locus carries recessive alleles (bb) in sugar beet (Beta vulgaris L. spp. vulgaris), so that vernalization and LDs are required for boltin...

Objective: To investigate the effect of lysozyme (LYZ) on invasion and migration of the human lung carcinoma A549 cells. Methods: The LYZ knockdown lentivirus (LV3-LYZ) was used to silence the expression of LYZ gene. GFP fluorescence detector and Western bolting were used to detect the efficiency and effectiveness of gene silencing. Effect of LYZ expression silence on invasion ability of the A5...

The natural variation in leaf and plant longevity in Arabidopsis thaliana was analysed in a set of 45 ecotypes and 155 recombinant inbred lines derived from a Cape Verde Islands (Cvi) x Landsberg erecta (Ler) cross. Post-bolting longevity was inversely related to time to flowering and rosette leaf number in the set of 45 ecotypes, with Cvi having the longest and Ler the shortest post-bolting lo...

MicroRNAs (miRNAs) play vital regulatory roles in plant growth and development. The phase transition from vegetative growth to flowering is crucial in the life cycle of plants. To date, miRNA-mediated flowering regulatory networks remain largely unexplored in radish. In this study, two small RNA libraries from radish leaves at vegetative and reproductive stages were constructed and sequenced by...

With the increasing complexity of engineering geological conditions, problem tensile-shear failure bolts is becoming more serious. Accurate modelling performance bolted rough joints subjected to load essential for support design. In this paper, based on theoretical criteria bolt failure, mechanical model Pile element in FLAC3D program modified, and accuracy modified verified by numerical experi...

A dynamic numerical method is established to simulate the stability of surrounding rocks retained roadway in FLAC3D, along with a double-yield constitutive model re-compaction process gangue and strain-softening characteristic coal grouted fragmented after yielding. The simulation reveals that grouting slurry diffusion range, mining affecting stage behind working face rib deformation are closel...

This paper proposes a new interface constitutive model for fully grouted rock-bolts and cable-bolts based on pull-out test results. A database was created combining published experimental data with in-house tests. By means of comprehensive framework, Coulomb-type failure criterion accounting friction mobilization defined. During the elastic phase, in which joint is not yet created, proposed pro...

In the process of numerical studies, stress-strain state rock massif around mine working driven at a depth 600 m was determined. The article presents analysis distribution total vertical and horizontal stresses isolines in roof, as well ratio residual strength rocks to initial. influence roof bolting on displacement contour established. Dangerous zones near-contour preparatory were identified. ...