Rumen microorganisms rapidly metabolized glycine at rates varying from 0.014 to 0.241 mumole of glycine per ml per min. The main metabolic products were carbon dioxide, acetic acid, and ammonia; little glycine was incorporated into bacterial protein. Use of carboxyl or methylene-labeled glycine showed that the carbon dioxide came mainly from the carboxyl of glycine, whereas both carbons of acet...

Meat and milk from ruminants are rich in saturated fat and this influences human health. The saturation of fat occurs in the rumen where microorganisms hydrogenate unsaturated fatty acids (FA). The metabolism of the FA in the rumen also influences the synthesis of FA in the mammary gland and can be responsible for a decrease in milk fat production. The purpose of this research is to identify th...

The manipulation of the rumen microbial ecosystem to improve animal productivity and lessen environmental impacts has been a research goal for many decades. Impediments to a targeted manipulation of the rumen have included a lack of knowledge of the diversity of the rumen microbial population as well as a lack of methods to thoroughly evaluate microbial changes. Molecular based studies of the r...

The nutritional manipulations of the rumen microbiome to enhance productivity and health are rather limited by the resilience of the ecosystem once established in the mature rumen. Based on recent studies, it has been suggested that the microbial colonization that occurs soon after birth opens a possibility of manipulation with potential to produce lasting effects into adult life. This paper pr...

Ureolytic bacteria are key organisms in the rumen producing urease enzymes to catalyze the breakdown of urea to ammonia for the synthesis of microbial protein. However, little is known about the diversity and distribution of rumen ureolytic microorganisms. The urease gene (ureC) has been the target gene of choice for analysis of the urea-degrading microorganisms in various environments. In this...

This study was conducted to compare digestibility of wheat straw (WS) by fungi and whole rumen microorganisms (WRM). Dry matter (DM), neutral and acid detergent fiber (NDF and ADF) digestibility of WS were compared with in vitro digestion (IVD), gas production (GP) and specific rumen anaerobic fungi culture (SRAFC). Dry matter, NDF and ADF digestibility of WS by WRM of buffalo (60.80, 49.93 and...