Rotavirus nonstructural glycoprotein NSP4 can induce diarrhea in newborn mice. It has been suggested that NSP4 may be a key determinant for rotavirus pathogenesis and a target for vaccine development. In order to study the biological and morphological role of NSP4 a large amount of the purified protein and antibody against it are required. Simian rotavirus SA11 was propagated in BSCl cell,...

The aim of this work was to find out the effects of Annona squamosa on the formation of glycosaminoglycans and collagen during wound healing in normal and diabetic rats. Diabetes induced rats were segregated into 4 groups, each containing six animals. Groups I and III served as the normal and diabetic control while groups II and IV served as normal and diabetic treated. The animals were treated...

An arylsulfatase (EC 3.1.6.1) was extracted from Pseudomonas aeruginosa PA01 and purified 2700fold to homogeneity. Synthesis of this enzyme was repressed when sulfate, cysteine or thiocyanate was supplied as the sole sulfur source for growth, but derepressed with all other sulfur sources tested. The apparent molecular mass was determined by SDSPAGE to be 57 kDa, and the enzyme was presumed to b...

Results Seventy five mg of allergen extract was loaded on to Q Sepharose column and adsorbed proteins were eluted using NaCl gradient. The eluted fractions with high intensity single band were pooled for further purification using Superdex 75 and C18column. In the major eluted peak a 31 kDa protein appeared as a single band on SDSPAGE after silver staining and was recognized by kidney bean hype...

This experiment was conducted to study the role of glyoxalase system in conferring salinity and drought stress in Brassica spp. Two Brassica genotypes viz. BARI Sharisha16 (tolerant) and Tori7 (susceptible) were exposed to salt (16 dS m) and drought for 2, 4 and 6 days. The comparative study of two Brassica genotypes under salinity and drought stresses revealed that BARI Sharisha16 is more tole...

Peroxidase catalyzes the oxidation of various electron donor substrates such as phenol and aromatic amines in the presence of hydrogen peroxide. In this study, peroxidase was purified 164-fold from the leaves of Moringa oleifera L. with a recovery of 28% by ammonium sulphate precipitation, DEAE-cellulose column chromatography, Sephadex G-200 column chromatography, and Con-A column chromatograph...

Glutathione S-transferases (GST) play an important role in the detoxification of many substances including organic pollutants and plant secondary metabolites. We compared the GST of two syrphid species, the aphidophagous Syrphus ribesii and the saprophagous Myathropa florea to assess the relation between feeding type and GST patterns. Differences between the GST of the hoverfly species were obs...

Background: Crude latex extracts are commonly used in skin prick tests (SPT) for the diagnosis of natural rubber latex (NRL) allergy. Nevertheless, variations in protein and allergen composition between latex extracts from different manufacturers can hamper a correct diagnosis. Objectives: To analyze the heterogeneity of proteins and allergens in latex extracts from 7 different manufacturers an...

Enzymatic properties of glutamate decarboxylase (GAD) [EC4.1.1.15] obtained from the fruiting body of Flammulina velutipes were evaluated via protein isolation, purification, and characterization. To investigate the anatomical localization of GAD, crushed fruiting bodies were centrifuged; the supernatant and precipitate were subsequently subjected to enzyme reaction. GAD activity of the precipi...

Lipases (Triacylglycerol acylhydrolase) are industrially important enzymes that are mainly used in the food and pharmaceutical industry. Lipase enzymes are also used in food materials, detergent, medicine, cosmetics, leather, perfumery and other items. The enzyme was isolated from Myroides odoratimimus and optimized to different physiochemical characteristics, giving its optimum temperature 37°...