CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...

fasciolosis diagnosis, due to low sensitivity of coprological diagnostic method has been challenging for a long period. in this study, dot-elisa, one of the simplest and the most sensitive tests in this regard, was evaluated using excretory-secretory antigens of fasciola hepatica to diagnose human fasciolosis three groups consisting of patients infected with fasciolosis (n= 95), patients with o...

In order to find correlations between skin gland morphology and specific ethological features, the cutaneous glands of the foot pads of Procavia capensis were studied by histological and various histochemical methods and by electron microscopy. In the foot pads, abundant specific eccrine skin glands occur, which consist of coiled tubular secretory portions and coiled ducts. The wall of the secr...

Rat liver microsomal membranes were purified in order to remove membrane-associated secretory products. Measurements of the decay of the newly synthesized protein of these membranes in vivo were carried out at short time intervals after the protein was labeled by the administration of radioactive leucine. The result of these measurements suggest that the membranes are synthesized and degraded a...

The sweat eccrine and apocrine glands of human skin secrete many functionally significant substances, including hydrolytic enzymes, onto its surface through the mechanisms secretion, excretion recruitment. Shown is their recreational origin as a secretory product digestive glands—pepsinogen by stomach glands, α-amylase—by pancreas. Hydrolases in epidermal barrier provide hydrolysis proteins, ca...

A rat medullary thyroid carcinoma cell line, CA-77, has been established as a model system for investigating calcitonin biosynthesis and secretion. Growth of this cell line in serum-free defined medium provided suitable conditions for studying steroid hormone effects on the production of calcitonin and related peptides. After exposure for 5 days to a variety of steroids, only dexamethasone and ...

CYP3A4 probably has the broadest catalytic activity of any cytochrome P450. It is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. Firstly, a total of 300 bp core distal enhancer of CYP3A4 XREM region (-7972/-7673) were amplified from human genomic DNA. The PCR product was then ligated into a human secretory alkaline phosphat...