Enhancement of salinity tolerance during seed germination is very important for direct seeding in rice. In this study, the salt-tolerant japonica landrace Jiucaiqing was used to determine the regulators that are involved in seed imbibition under salt stress. Briefly, the comparative proteomic analysis was conducted between dry (0 h) and imbibed (24 h) seeds with 150 mM NaCl. Under salt stress, ...

Seed storage reserves mainly consist of starch, triacylglycerols, and storage proteins. They not only provide energy for seed germination and seedling establishment, but also supply essential dietary nutrients for human beings and animals. So far, the regulatory networks that govern the accumulation of seed storage reserves in plants are still largely unknown. Here, we show that TRANSPARENT TES...

this study was performed in order to evaluate the effects of irrigation and application of zn fertilizer on  seed storage proteins  in chickpea, azad cultivar. experiment was performed in an factorial using randomized complete block design with three replications. in this experiment, two factor consist of irrigation in three levels (i1= non irrigation and i2= irrigation in flowering stage), zn ...

A high-throughput proteomic approach was employed to determine the expression profile and identity of hundreds of proteins during seed filling in soybean (Glycine max) cv Maverick. Soybean seed proteins were analyzed at 2, 3, 4, 5, and 6 weeks after flowering using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. This led to t...

to better understand seed priming that improve seed germination and seedling uniformity, we examined the physiological role of osmopriming in germination of bread wheat (triticum aestivum l.) seed embryos by a proteomic approach. for this purpose, we used two systems. the first system consisted of osmo-primed seeds by peg 6000 at -0.8 mpa at 15?c for 24 hours, followed by air drying at 15?c, an...

Seed development culminates in programmed cell death (PCD) and hardening of organs enclosing the embryo (e.g., pericarp, seed coat) providing essentially a physical shield for protection during storage in the soil. We examined the proposal that dead organs enclosing embryos are unique entities that store and release upon hydration active proteins that might increase seed persistence in soil, ge...

Identification of anonymous proteins from two-dimensional (2-D) gels by peptide mass fingerprinting is one area of proteomics that can greatly benefit from a simple, automated workflow to minimize sample contamination and facilitate high-throughput sample processing. In this investigation we outline a workflow employing robotic automation at each step subsequent to 2-D gel electrophoresis. As p...

Brassica napus (cultivar Reston) seed proteins were analyzed at 2, 3, 4, 5, and 6 weeks after flowering in biological quadruplicate using two-dimensional gel electrophoresis. Developmental expression profiles for 794 protein spot groups were established and hierarchical cluster analysis revealed 12 different expression trends. Tryptic peptides from each spot group were analyzed in duplicate usi...

Seed proteins of eight species of the genus Suaeda (Chenopodiaceae) were compared by means of polyacrylamide disc gel electrophoresis. Different samples of the same species showed not less than 89% similarities, and geographical separation had no remarkable effect on seed protein patterns of the same species. Morphological heterogenity, that creates difficulties in infrageneric classificat...