A high-throughput proteomic approach was employed to determine the expression profile and identity of hundreds of proteins during seed filling in soybean (Glycine max) cv Maverick. Soybean seed proteins were analyzed at 2, 3, 4, 5, and 6 weeks after flowering using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry. This led to t...

The plant seed is not only an organ of propagation and dispersal but also the major plant tissue harvested by humankind. The amount of protein present in seeds varies from -10% (in cereals) to -40% (in certain legumes and oilseeds) of the dry weight, forming a major source of dietary protein. Although the vast majority of the individual proteins present in mature seeds have either metabolic or ...

Seed development culminates in programmed cell death (PCD) and hardening of organs enclosing the embryo (e.g., pericarp, seed coat) providing essentially a physical shield for protection during storage in the soil. We examined the proposal that dead organs enclosing embryos are unique entities that store and release upon hydration active proteins that might increase seed persistence in soil, ge...

Allergic reactions to fruits and vegetables are frequently observed, however skin prick tests and serological analyses may not always confirm allergy. Recent advances have identified the seeds as potential allergen sources. Seeds are generally rich in storage proteins like 7S/11S globulins and 2S albumins, which are allergens known to be linked to severe reactions. For a number of plant food, s...

Pistachio is one of the most important agricultural products that have always been associated with Iran, and its production has a long historical background in our country. In this research, protein patterns of 10 cultivars of Pistacia vera L. were compared in which cultivars grown in normal conditions where compared with cultivars grown in salinity and water shortage to determine diversity. Fo...

We have measured the numbers of genes coding for the three seed storage proteins, vicilin, convicilin and legumin, in a number of Pisum genotypes of variant protein composition. No difference in gene number existed among P. sativum genotypes for any of the proteins. There were differences in the number of genes coding for individual proteins with approximately 11 genes (per haploid genome) for ...

The critical node (CN), which is the transition from the plateau phase to the rapid decreasing phase of seed ageing, is extremely important for seed conservation. Although numerous studies have investigated the oxidative stress during seed ageing, information on the changes in protein abundance at the CN is limited. In this study, we aimed to investigate the abundance and carbonylation patterns...