نتایج جستجو برای: sequence specific primers ssp
تعداد نتایج: 1388869 فیلتر نتایج به سال:
Background. Microlymphocytotoxicity (MLCT) and flowcytometry (FC) are the conventional serological methods to detect HLA-B* 27. Due to some disadvantages in these methods, most of the HLA laboratories have now switched over to molecular methods. Molecular techniques based on commercial kits are expensive; as such many laboratories with limited funds in developing countries cannot afford these t...
objective: this study aimed to investigate the association between hla alleles and visceral leishmaniasis (vl) in a sample of iraqi patients. methods: a total of 30 patients were studied, in addition to 20 age, gender and ethnicity matched controls. all subjects were genotyped by polymerase chain reaction-sequence specific primers (pcr-ssp) method. results: for hla-class i region (a and b loci)...
Abstract Background Migraine is a neurovascular disorder; several studies have demonstrated the immune system plays key role in migraine pathogenesis . The aim of this study was to investigate association between FOXP3 gene polymorphism and susceptibility migraine. Methods In case–control study, 55 whole blood samples patients with 80 healthy were collected. After DNA extraction, genotyping rs5...
wild grasses are the most important primary feedstuffs which are susceptible to contamination with toxigenic fungi belonging to aspergillus spp. in order to explore diversity of aspergillus species associated with the inflorescences of gramineous weeds, infected inflorescences were collected from wild grasses in western parts of iran. fifty-six aspergillus isolates were obtained from all diseas...
Genetic relationship of a newly discovered teosinte from Nicaragua, Zea nicaraguensis with waterlogging tolerance, was determined based on randomly amplified polymorphic DNA (RAPD) markers and the internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA using 14 accessions from Zea species. RAPD analysis showed that a total of 5,303 fragments were produced by 136 random decamer prim...
It is widely accepted that donor leukocytes survive within the recipient periphery after blood transfusion or solid organ transplantation. The significance of this microchimerism remains unclear, partially because of the insecurity of assays used to detect the donor-derived material. The techniques used to detect donor-derived DNA within recipient peripheral blood rely largely on major histocom...
BACKGROUND Duffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequence-specific primers (PCR-SSP), and the probability of obtaining compatible bl...
Knowledge of allele frequencies of the human leukocyte antigen (HLA) system is essentially important in the search for unrelated kidney and bone marrow donors. The Romanian Caucasian population is heterogeneous and the HLA genotyping of the most common alleles brings information on HLA polymorphisms. We have characterized the HLA genetic profile of regional populations in Romania. By this we ha...
This article documents the addition of 139 microsatellite marker loci and 90 pairs of single-nucleotide polymorphism sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Aglaoctenus lagotis, Costus pulverulentus, Costus scaber, Culex pipiens, Dascyllus marginatus, Lupinus nanus Benth, Phloeomyzus passerini, Podarcis muralis, Rhododendron...
The genus Harpagophytum has two species: H. procumbens which is an important medicinal plant in southern Africa, and H. zeyheri. Genetic diversity in 96 samples, obtained by germinating seeds collected from Botswana, was assessed using six inter-simple sequence repeat (ISSR) and 10 random amplified polymorphic DNA (RAPD) primers. These DNA markers yielded a total of 138 polymorphic bands. Polym...
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