Analysis of extracts obtained from shoots, roots and exudates of Brassica alba revealed the presence of 3,5,6,7,8-pentahydroxy-4'-methoxy flavone in shoots, as well as 2',3',4',5',6'-pentahydroxy chalcone and 3,5,6,7,8-pentahydroxy flavone in roots and exudates. Apigenin was also found in the shoots and roots, but not in the root exudates.

In vitro multiple shoots, root, callus and cell suspension cultures of Withania somnifera exhibited the potentiality to produce pharmacologically active withanolides. Multiple shoots cultures exhibited an increase in withanolide A accumulation compared to shoots of the mother plant. In vitro generated root cultures as well as callus and suspension cultures also produced withanolides albeit at l...

Cotyledonary nodes taken alongwith shoot apex from seedlings of cotton (G. hirsutum) proliferated into shoots on nutrient agar medium supplemented with cytokinins. In the presence of optimal plant growth regulators, low light intensity enhanced the number of shoots initiated per explant in cotton. An average of 33.5 +/- 2.9 shoots were obtained from a single explant cultured for 8 weeks which i...

The performance of canola (Brassica napus L.) was evaluated using various nitrogen levels and shoots cutting at New Developmental Farm of The University of Agriculture, Peshawar, Pakistan during Rabi 2012-13. The experiment was laid out in randomized complete block design (RCBD) with split plot arrangement having four replications each. Nitrogen levels @ 0, 50, 75 and 100 kg ha were allotted to...

Cytokinin flow from roots to shoots can serve as a long-distance signal important for root-to-shoot communication. In the past, changes in cytokinin flow from roots to shoots have been mainly attributed to changes in the rate of synthesis or breakdown in the roots. The present research tested the possibility that active uptake of cytokinin by root cells may also influence its export to shoots. ...

In our laboratory, bacterial contamination was detected in cultures of Pelargonium x hederaefolium ‘Bonete’ that had been subcultured continuously for several years. Two contaminating bacteria were isolated and identified by partial sequence analysis of 16S rRNA gene as Paenibacillus glycanilyticus and Lactobacillus paracasei. Antibiotic sensitivity testing was performed on the bacterial isolat...

Transgenic plants harboring the left transfer DNA (T(L)-DNA) of the root inducing plasmid of Agrobacterium rhizogenes show many developmental abnormalities. We observed frequent appearance of normal looking lateral (revertant) shoots from such aberrant plants. Unlike aberrant shoots of the plant, revertant shoots exhibited a very high growth rate and set viable seeds. Sexual and vegetative repr...

nut (Juglans regia L.) shoots by subculturing the shoots on rooting medium containing agar and 3 mg l indole-3-butyric acid (IBA) for 7 days in darkness. Changes in the concentrations of endogenous free indole-3-acetic acid (IAA), indole-3acetylaspartic acid (IAAsp) and free polyamines were determined during culture on root-inducing medium. In extracts of whole shoots, the concentration of free...