The aim of the present study is to rationalize acrylamide pendant Phos-Tag™ in-gel discrimination of phosphorylated and non-phosphorylated plant protein species with standard immunoblot analysis, and optimize sample preparation, efficient electrophoretic separation and transfer. We tested variants of the method including extraction buffers suitable for preservation of phosphorylated protein spe...

Purpose To assess the predictive accuracy of simulation-based LASIK outcomes. Methods Preoperative and 3-month post-LASIK tomographic data from 20 eyes of 12 patients who underwent wavefront-optimized LASIK for myopia were obtained retrospectively. Patient-specific finite element models were created and case-specific treatment settings were simulated. Simulated keratometry (SimK) values and t...

Purpose: This study aims to elucidate the factors contributing temporal decentration of orthokeratology lens (OK lens) treatment zone, employing profiles obtained from anterior segment swept-source optical coherence tomography (AS SS-OCT).Methods: The measurements prior were taken using AS SS-OCT (Anterion; Heidelberg, Germany) in subjects who had worn OK lenses for at least 2 weeks. collected ...

BACKGROUND As new methods for corneal curvature measurement have evolved, users of videokeratscopes need to know the practical limitations of these instruments. We assessed agreement between keratometry and videokeratography in measuring highly astigmatic corneas. METHODS Two independent examiners made three keratometric and videokeratographic measurements on each of 33 corneas after penetrat...

purpose: to evaluate agreement between galilei, orbscan ii, and placido disk-based topography in terms of keratometry readings, and anterior and posterior elevation. methods: in this prospective comparative study, 184 eyes of 92 keratorefractive surgery candidates were evaluated with galilei, orbscan ii, and placido disk-based topography. keratometry readings and anterior and posterior elevatio...

In this paper we propose a new tool called OWL-CM (OWL Combining Matcher) that deals with uncertainty inherent to ontology mapping process. On the one hand, OWL-CM uses the technique of similarity metrics to assess the equivalence between ontology entities and on the other hand, it incorporates belief functions theory into the mapping process in order to improve the effectiveness of the results...

AIMS To map the thickness, elevation (anterior and posterior corneal surface), and axial curvature of the cornea in normal eyes with the Orbscan corneal topography system. METHODS 94 eyes of 51 normal subjects were investigated using the Orbscan corneal topography system. The anterior and posterior corneal elevation maps were classified into regular ridge, irregular ridge, incomplete ridge, i...