Size exclusion chromatography (SEC) is widely used for molecular weight estimations of proteins in their native state. SEC has found applications in studies on protein purity, protein-protein interactions and protein aggregation. The major limitations of SEC are that the retention time of proteins can be affected by their hydrodynamic properties and also due to tendency of some proteins to inte...

In the characterization of polymers, size-exclusion chromatography (SEC) has become a standard technique for determining molar mass averages andmolar mass distributions (MMD) of polymers. The principle of SEC is easily understood: owing to limited accessability of the pore volume within the particles of the column packing, polymer molecules are separated according to their hydrodynamic volumes,...

MicroRNAs (miRNAs) encapsulated in extracellular vesicles (EVs) are potential diagnostic and prognostic biomarkers. However, discrepancies miRNA patterns their validation still frequent due to differences sample origin, EV isolation, sequencing methods. The aim of the present study is find a reliable isolation method for sequencing, adequate clinical application. To this aim, two comparative st...

High-performance liquid chromatography (HPLC) with UV, circular dichroism (CD) and intrinsic fluorescence detection was applied to monitor conformational properties of recombinant human interferon alpha2b when performing size exclusion chromatography (SEC) and reversed-phase HPLC (RP-HPLC). In this way native conditions during SEC and structural changes of the protein during RP-HPLC were demons...

Biological small angle X-ray scattering (BioSAXS) is a powerful technique in molecular and structural biology used to determine solution structure, particle size and shape, and surface-to-volume ratio of macromolecules. The technique is applicable to a very wide variety of solution conditions spanning a broad range of concentrations, pH values, ionic strengths, temperatures, additives, etc., bu...

We have found that the concentrated poly(methyl methacrylate) (PMMA) brush showed the very good nanoparticles (NPs) repellency in its good solvent, e.g. tetrahydrofuran (THF). Whereas the oil- and hydro-phobic (fluorinated), hydrophobic and hydrophilic surfaces adsorbed a lot of NPs. The repellency of NPs did not depend on the surface nature of the NPs. Preparing absorption free columns for siz...

Extracellular inulinase (E.C. 3.2.1.7) produced by Aspergillus niger 20 OSM culture in a 2-litre fermentor was isolated and purified by ion exchange chromatography on DEAE Sepharose, hydrophobic interaction on phenyl Sepharose, chromatofocusing on PBE-94, and size exclusion chromatography (SEC) on Sephadex G-200. The enzyme was homogeneous, as measured by SDS-PAGE with an apparent molecular mas...