o. There are two different cryopreservation protocols used in skin preservation: slow freezing and quick freezing/snap frozen. Slow freezing can be achieved using a controlled-rate cooling apparatus (Blondet et al., 1982). There is an optimum cooling rate for any cell type that produces maximum cell survival. On either side of this optimum, the survival rate falls. In the presence of CPA, a coo...

When a graft is placed on a recipient bed, the process of accepting the graft begins. For some hours, the graft is bathed and nourished by plasmatic circulation or serum imbibition. Si‐ multaneously, fortuitous and accidental apposition of the vessels in the bed and those in the graft allows blood to be sucked into the graft. Soon afterwards, active penetration of the graft by blood vessels fro...

Although skin grafting originated 2500 to 3000 years ago, it was until the 19 th century that this technique was again introduced as a reconstructive option. While 19th century surgeons used grafts to repair their most difficult cases, skin grafting has since evolved into a modality that is routinely and sometimes preferentially used for the surgical repair of skin defects [1]. In this chapter ...

Background. Hyperbaric oxygen (HBO) therapy, which is used for many conditions, may also have immunosuppressive effects and could be used for prevention or treatment of graft-versus-host disease (GvHD). If HBO is immunosuppressant, then we hypothesize that HBO therapy will delay the T-cell mediated skin graft rejection. Methods. C57/BL6 black-coated (H2B) mice received skin graft from CBA (H2D)...

BACKGROUND The treatment of chronic lesions in hidradenitis suppurativa (HS) remains a challenge for dermatologists. In most cases, wide surgical excision of the affected skin reduces the recurrence rate to a minimum. Split-thickness skin grafts have usually been applied to resurface large postoperative defects. OBJECTIVE. : The aim of this study is to introduce an alternative method of skin gr...

tissue-engineered gingival graft was used for regenerating facial gingiva around an implant at lower left first premolar area with insufficient attached gingiva. engineered gingival graft was produced by mixing 250 ml bovine skin collagen with 250 ml nutritional medium containing human gingival fibroblasts (2×105). 3 months post-surgery, there were gains in the attached gingiva compared to pre-...

Reconstruction of any soft tissue defect is managed as per conventional reconstructive ladder. The options range from healing by secondary intention, primary closure, skin graft and flaps. Skin graft is one of the commonest methods of reconstruction. For proper take of skin graft, the graft needs to be adherent to the wound bed for first few days. The fixation should not allow any hematoma or s...

The participation of regulatory T (Treg) cells in B cell-induced T cell tolerance has been claimed in different models. In skin grafts, naive B cells were shown to induce graft tolerance. However, neither the contribution of Treg cells to B cell-induced skin tolerance nor their contribution to the histopathological diagnosis of graft acceptance has been addressed. Here, using male C57BL/6 naive...