(1) We have measured the incompatible phase separation that occurs in a polyethylene glycol-sodium dextran sulphate-sodium chloride-water system and have determined a critical point. (2) We have measured the activity coefficients of sodium chloride in critical-point concentrations of polyethylene glycol and sodium dextran sulphate respectively, and the osmotic coefficient of sodium dextran sulp...

evaluation of the geochemistry and hydrochemical quality of bam salt dome located in southern iran, was conducted in this study. two composite samples from salt units were collected and analysed by xrd and xrf to determine their mineral and elemental compositions. water samples were also collected from the only spring in the area and analysed for major anions, cations and some toxic elements. t...

the thermal denaturation of adenosine deaminase (ada) has been investigated in the presence of sodium n-dodecyl sulphate (sds) over the temperature range of (293-363k) in 2.5 mm phosphate buffer, ph 6.4 by temperature scanning spectroscopy. the interaction of sds caused the folding of adenosine deaminanse resulting in a decrease of th (temperature of minimum solubility), ts (temperature of maxi...

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to identify species-specific water soluble proteins and salt soluble proteins bands of raw fillets, surimi, sausage and different combinations (1:1; 3:1; 1:3; and 4:1) of surimi mixtures; sausage mixtures from the two species of fish, threadfin bream (Nemipterus japonicus), and bulls-eye (Priacanthus hamrur). 7.5% SD...

The role of intermediates in the folding reaction of single-domain proteins is a controversial issue. It was previously shown by different methods that an on-pathway intermediate is populated in the presence of sodium sulphate during the folding of the FF domain from HYPA/FBP11. Here we demonstrate using analysis of the amplitudes of kinetic traces that this burst-phase folding intermediate is ...

. The kinetics of the reaction between potassium persulphate and potassium iodide has been studied iodometrically in aqueous solutions of sodium dodecyl sulphate (SDS) at various surfactant concentrations. It is observed that from low concentrations up to the c.m.c, the surfactant behaves like a normal electrolyte exhibiting primary kinetic salt effect. In presence of micelles it is observed th...

1. N-Acetylgalactosamine 6-sulphate sulphatase was purified about 20000-fold from the soluble extract of human placenta with N-acetylgalactosamine 6-sulphate-glucuronic acid-N-acetyl[1-(3)H]galactosaminitol 6-sulphate as substrate in the activity assay. The enzyme appears to be a glycoprotein with a mol.wt. of about 100000 as determined by gel filtration. On gel electrophoresis in the presence ...

Abstract Steady-state fluorescence measurements were used to examine the quenching of 1, 4-bis [-(2-benzothiazolyl) vinyl benzene (BVB) by sodium salt meso-tetrakis (4-sulfonatophenyl) porphyrin (TPPS) in presence and absence silver nanoparticles (Ag NPs). The energy transfer (ET) process’s emission intensities Stern–Volmer constants (K SV ) showed that Ag NP’s increased ET’s efficiency. molecu...

ABSTRACT The study of thermal properties frozen salt solutions representative ice layers in Jovian moons is crucial to support the JUpiter ICy Explorer (JUICE) (ESA) and Europa Clipper (NASA) missions, which will be launched upcoming years make detailed observations giant gaseous planet Jupiter three its largest (Ganymede, Europa, Callisto), due scarcity experimental measurements. Therefore, we...

Alkaline phosphatase from human liver was purified to homogeneity. The purification procedure included solubilization with butanol, fractionation with acetone, and chromatography on concanavalin A-Sepharose, DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex. Purity was established by standard and sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. The isoelectric point of the protein was...