A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of t...

In the soybean (Glycine max (L.) Merr.), the gene combination Fg1 Fg3 is responsible for the glycosylation in the biosynthesis of kaempferol triglucoside (K9) in leaves. The presence of K9 is associated with reduction in chlorophyll content, specific leaf mass, photosynthetic rate and stomatal frequency. Blocking the action of Fg1 Fg3 with the magenta flower gene wm prevents formation of K9 and...

Characterization of nodule growth and functioning, phosphorus status of plant tissues and hostplant growth of nodulated soybean (Glycine max L. Merr.) plants grown under different phosphorus conditions was studied in order to evaluate the role of phosphorus in symbiotic nitrogen fixation. Phosphorus deficiency treatment decreased the whole plant fresh and dry mass, nodule weight, number and fun...

Three lethal-yellow mutants have been identified in soybean (Glycine max (L.) Merr.), and assigned genetic type collection numbers T218H, T225H, and T362H. Previous genetic evaluation of T362H indicated allelism with T218H and T225H and duplicate-factor inheritance. Our objectives were to confirm the inheritance and allelism of T218H and T225H and to molecularly map the locus and (or) loci cond...

Soybean cyst nematode (Heterodera glycines, SCN) is the most harmful pathogen of soybean (Glycine max (L.) Merr.) worldwide. In 2016, a new soybean-parasitic nematode, Heterodera sojae (the white nematode) was found parasitizing roots plants in Korea. To investigate distribution and population density H. sojae, 943 soil samples were collected from fields all nine provinces Korea 2017 to 2018. C...

Soybean (Glycine max (L) Merr.) seed treatments are recognized as useful in reducing losses from seed borne pathogens and seedling damping off agents. The seed treatment with fungicide is essential in the improved technology for increasing crop production. In the present investigation two seed protectant fungicides captan and carbendazim are used. Effect of fungicides on growth of Rhizobium jap...

While the cultivated soybean, Glycine max (L.) Merr., is more recalcitrant to pod dehiscence (shattering-resistant) than wild soybean, Glycine soja Sieb. & Zucc., there is also significant genetic variation in shattering resistance among cultivated soybean cultivars. To reveal the genetic basis and develop DNA markers for pod dehiscence, several research groups have conducted quantitative trait...