Recently, an S haplotype-specific F-box (SFB) gene has been proposed as a candidate for the pollen-S specificity gene of RNase-mediated gametophytic self-incompatibility in Prunus (Rosaceae). We have examined two pollen-part mutant haplotypes of sweet cherry (Prunus avium). Both were found to retain the S-RNase, which determines stylar specificity, but one (S3' in JI 2434) has a deletion includ...

∗This work was financially supported by the Austrian Science Foundation (FWF) grant SFB F013 and the Christian Doppler Research Association (CDG). S. Gerhold gratefully acknowledges a fruitful collaboration and continued support by the Austrian Federal Financing Agency and Bank Austria through CDG. M. Kauers was supported by the Austrian Science Foundation FWF under SFB F013 grant number F1305 ...

This paper1 deals with the definition of a computational semantics for spatial relations between objects in a scene. In particular we examine the effects of the observer's position on the computation of spatial relations, the use of path prepositions, and degrees of applicability for spatial relations. Representational prerequisites and the computational analysis of spatial relations are invest...

Environmental toxicants such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an aryl hydrocarbon receptor (AhR), are known to induce host toxicity and structural shifts in the gut microbiota. Key bacterial populations with similar or opposing functional responses to AhR ligand exposure may potentially help regulate expression of genes associated with immune dysfunction. To examine this question ...

In the field of positron emission tomography (PET) radiochemistry, compact microreactors provide reliable and reproducible synthesis methods that reduce the use of expensive precursors for radiolabeling and make effective use of the limited space in a hot cell. To develop more compact microreactors for radiosynthesis of 18F-labeled compounds required for the multistep procedure, we attempted ra...

To reduce culture artifacts by conventional repeated passaging and long-term culture in vitro, the isolation of synovial fibroblasts (SFB) was attempted from rheumatoid arthritis (RA) synovial membranes by trypsin/collagenase digest, short-term in vitro adherence (7 days), and negative isolation using magnetobead-coupled anti-CD14 monoclonal antibodies. This method yielded highly enriched SFB (...

Segmented filamentous bacteria (SFB) are commensal bacteria first identified in the ileum of mice and rats. Morphologically similar bacteria have been reported in a broad range of host species, however all strains have been refractory to in vitro culture thus far. While SFB were once considered innocuous members of the intestinal microbiota of laboratory rodents, they have now been shown to imp...

IL-17-expressing CD4+ T lymphocytes (Th17 cells) naturally reside in the intestine where specific cytokines and microbiota, such as segmented filamentous bacteria (SFB), promote their differentiation. Intestinal Th17 cells are thought to initially differentiate in the GALT and/or mesenteric lymph nodes upon Ag encounter and subsequently home to the lamina propria (LP) where they mediate effecto...

RORγt(+) Th17 cells are important for mucosal defenses but also contribute to autoimmune disease. They accumulate in the intestine in response to microbiota and produce IL-17 cytokines. Segmented filamentous bacteria (SFB) are Th17-inducing commensals that potentiate autoimmunity in mice. RORγt(+) T cells were induced in mesenteric lymph nodes early after SFB colonization and distributed across...

This Annual Report gives a summary of SFB results achieved in 2005. Also in its eighth year of funding, the overall scientific goal of the SFB is the design, verification, implementation , and analysis of numerical, symbolic, and geometrical methods for solving • large-scale direct and inverse problems with constraints and their synergetical use in scientific computing for real life problems of...