The human leukemia cell lines HL-60, KG-1, KLM-2, ML-3, THP-1, and U-937 were treated with the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA). TPA partially or completely inhibited the proliferative activity of the cell cultures. The number of cells with the ability to reduce nitroblue tetrazolium increased in the TPA-treated cell lines HL-60, ML-3, THP-1, and U-937, whereas the cell ...

Mitochondria mediate diverse cellular functions including energy generation and ROS (reactive oxygen species) production and contribute to signal transduction. Mitochondria are also key regulators of cell viability and play a central role in necrotic and apoptotic cell death pathways induced by cardiac ischaemia/reperfusion injury. PKC (protein kinase C) ε plays a critical role in cardioprotect...

The clinical application of 84 prostate specific antigen (PSA) and prostatic acid phosphatase (PAP) measurements for the follow-up of 36 patients with treated prostate cancer was retrospectively examined by case study. Clinicians use several risk markers including serum levels of PSA and PAP to monitor prostate cancer progression or stability. These results of PAP and PSA tests were either util...

background: leishmaniasis is endemic in iran. different species of leishmania (l.) parasites are causative agents of this disease. correct identification of leishmania species is important for clinical studies, prevention, and control of the diseases. mix up of leishmania isolates is possible in the laboratory, so there is need for verification of species for isolates of uncertain identity. dif...

during the period from 1996 to 1997, 45 isolates of leishmania were recovered frompatients suspected of cutaneous leishmaniasis (cl) and one from a gerbil. these specimens werereceived from various parts of iran. isoenzyme profiles of these isolates were compared with those ofreference strains of l.tropica, l.major and l.infantum using cellulose acetate electrophoresis (cae) and10 enzyme system...

Introduction Protein kinase C (PKC) is a family of phospholipiddependent serine-threonine kinases that regulate cell growth and differentiation (reviewed in [ 1 4 ) . PKC is also the major intracellular receptor for tumour-promoting phorbol esters [ 1-31. Molecular cloning and biochemical separation of the individual gene products has led to a classification system for the PKC isoenzymes based ...

Two ammonium-inducible, chloroplast-localized NADP-specific glutamate dehydrogenase isoenzymes were purified to homogeneity from Chlorella sorokiniana. These isoenzymes were homopolymers of either alpha- or beta-subunits with molecular weights of 55,500 or 53,000, respectively. The alpha-isoenzyme was preferentially induced at low ammonium concentrations (2 millimolar or lower), whereas only th...