A simple and rapid microextraction procedure based on the application of magnetic nanoparticles and hydrophobic 1-Hexylpyridinium hexafluorophosphate [Hpy][PF6] ionic liquid (IL) was applied to trace determination of gatifloxacin. A mixture of [Hpy][PF6] and magnetic nanoparticles was injected into the sample solution containing the analyse of interest and a cloudy solution was forme...

A simple and rapid microextraction procedure based on the application of magnetic nanoparticles and hydrophobic 1-Hexylpyridinium hexafluorophosphate [Hpy][PF6] ionic liquid (IL) was applied to trace determination of gatifloxacin. A mixture of [Hpy][PF6] and magnetic nanoparticles was injected into the sample solution containing the analyse of interest and a cloudy solution was forme...

BACKGROUND Neuroserpin, a member of the Serine Proteinase Inhibitor (Serpin) superfamily, is known to be a neuroprotective factor in the focal ischemic stroke followed by reducing the microglial activation. Neuroserpin is a protein rich of methionine residues that can scavenge the free radical species which may increase its neuroprotective effect. On the other hand, the oxidative modifications ...

Peritoneal cells from thioglycollate-stimulated mice were allowed to adhere to coverglasses for 2 h to give a dense monolayer of adherent cells greater than 95% of which were macrophages. After incubation with the tetra-acetoxymethyl ester of quin2, coverglasses were rinsed with Ca2+-free saline, oriented at a 45 degree angle in square cuvettes containing a magnetically driven stir bar, and ana...

BACKGROUND MK 886, a 5-lipoxygenase inhibitor, induces a type 1 "apoptotic" form of programmed cell death in Bcl-2-positive U937 monoblastoid cells. In Ca2+-depleted, nonpermeabilized U937 cells studied with MK 886 in a Ca2+-free medium, an acute increase in Ca2+ occured within 10 to 20 seconds, detected with fura-2 measured with a spectrofluorimeter. METHODS AND RESULTS The increased fluores...

The use of fluorescence as an analytical technique in clinical biochemistry and pathology is in the process of developing and it is therefore important that those who use it should understand the underlying principles governing the phenomenon of fluorescence and its application, so that this elegant and sensitive technique can be used to its greatest advantage. Fluorescence has been studied by ...

Neuroserpin, a member of the Serine Proteinase Inhibitor (Serpin) superfamily, is known to be a neuroprotective factor in the focal ischemic stroke followed by reducing the microglial activation. Neuroserpin is a protein rich of methionine residues that can scavenge the free radical species which may increase its neuroprotective effect. On the other hand, the oxidative modifications of the amin...

neuroserpin, a member of the serine proteinase inhibitor (serpin) superfamily, is known to be a neuroprotective factor in the focal ischemic stroke followed by reducing the microglial activation. neuroserpin is a protein rich of methionine residues that can scavenge the free radical species which may increase its neuroprotective effect. on the other hand, the oxidative modifications of the amin...

The M1 alanyl aminopeptidase from Plasmodium falciparum has been shown to be an essential hemoglobinase enzyme, catalyzing the final stages of hemoglobin break-down within intra-erythrocytic parasites 1. Recently there has been much interest in this protease as a potential drug target for the development of novel antimalarials [1-13]. In a recent report, Krishnamoorthy and Achary propose that S...

L-3-Hydroxyacyl coenzyme A dehydrogenase (L-3hydroxyacyl-CoA:NAD oxidoreductase; EC 1 .l .1.35) is a mitochondrial enzyme involved in the beta oxidation of fatty acids. The enzyme, as isolated from pig heart muscle, hasM, 67 000 and contains two identical subunits [l]. The amino acid sequence has recently been completed and appears to have little homology with other dehydrogenases [2]. Like man...