The synthesis of long-arm thiolated beads from beaded polyacrylamide ( $400 mesh, 10-37^ M diameter, 2,000 Dalton inclusion limit) is described. The long spacer arm, polyethylene glycol di(3-mercapto-propionate)(PEG-DMP) was i introduced via thioether formation with haloacetamidoethyl beads. The latter were prepared via the N-hydroxysuccinimide ester (NHS) after surveying a number of peptide co...

The reaction between phenothiazine and 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in the presence of p-toluenesulfonic acid in chloroform was investigated by potentiometry, voltammetry and spectrophotometry, and the reaction pathways were proposed. The conditions for the oxidimetric titration of phenothiazines with a standard chloroform solution of DDQ were optimized and the potentiometric...

Two titrimetric and two spectrophotometric methods are described for the assay of famotidine (FMT) in tablets using N-bromosuccinimide (NBS). The first titrimetric method is direct in which FMT is titrated directly with NBS in HCl medium using methyl orange as indicator (method A). The remaining three methods are indirect in which the unreacted NBS is determined after the complete reaction betw...

In this study, the development of an indirect spectrophotometric method for determination folic acid in pure and pharmaceutical preparations is described. The based on oxidation pyrocatechol with iron (III) acidic medium, followed by reaction (FA) to produce a stable, water-soluble orange compound maximum absorption at 350 nm versus blank reagent. complex charge transfer was studied under optim...

NADPH-cytochrome P-450 reductase was isolated from liver microsomes of phenobarbital-induced rats. The enzyme exhibits an apparent minimal molecular weight of 76,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contains 1 molecule each of FMN and FAD. Trypsin treatment of the reductase yields an enzyme with an apparent minimal molecular weight of 69,000 which r...

The interaction of bovine serum albumin ( BSA) and egg albumin with water solublephthalocyanine, cobalt (ΙΙ) 4, 4′ , 4′′, 4′′′- tetrasulfophthalocyanine ( CoTSPc) , has been studiedby the UV- Vis method at pH 7.0 and five different temperatures 20, 25, 30, 35 and 40°C. Theformation constants have been elucidated by using spectrophotometric titration and computerSQUAD program data refinement. Th...

Spectrophotometric and electrochemical studies were successfully used in quantitative analysis of Amitriptyline Hydrochloride (ATH) and Nortriptyline Hydrochloride (NTH). Conductometric titration were carried out in equeous solution using iodide and dopamine and serotonine as titrant. Two new methods using spectrophotometry are dascribed for the determination of (ATH) and (NTH) with potassium b...

Several esters of 4-methylumbelliferone and 2-naphthol were synthesized and examined as possible spectrofluorimetric titrants for bovine alpha-chymotrypsin, trypsin, thrombin, Factor Xa and for subtilisin Novo. 4-Methylumbelliferyl p-guanidinobenzoate hydrochloride (MUGB) is a satisfactory titrant for alpha- and beta-trypsin, thrombin and Factor Xa and 4-methylumbelliferyl p-(NNN-trimethylammon...

______________________________________________________________________________ In this paper the conductometric titration of propranolol hydrochloride in pharmaceutical formulations using silver nitrate as titrant is proposed. The method was based on the formation of an insoluble salt (AgCl(s)) between the chloride of propranolol hydrochloride molecule and Ag(I) ions of the titrant AgNO3. The e...

NADPH-cytochrome P-450 reductase was isolated from liver microsomes of phenobarbital-induced rats. The enzyme exhibits an apparent minimal molecular weight of 76,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and contains 1 molecule each of FMN and FAD. Trypsin treatment of the reductase yields an enzyme with an apparent minimal molecular weight of 69,000 which r...