Objective(s): Melatonin, an important hormone secreted by the epiphysis, is a powerful anti-oxidant with a high potential to neutralize medical toxins. The goal of this study was to demonstrate the beneficial effect of melatonin on epididymal sperm and reproductive parameters in mice treated with acetylsalicylic acid (ASA). Materials and Methods: Male adult mice were divided into four treatment...

We explored the relationship between sperm chromatin integrity, hormone levels, seminal plasma total antioxidant capacity (TAC), and routine sperm parameters in men with male factor (MF, n = 81) and non-male factor (NMF, n = 52) infertility. Semen and blood were collected and examined from men undergoing evaluation for infertility in the Avicenna Infertility Clinic. We have examined each patien...

introduction: semen parameters and specially sperm morphology is used for the assessment of semen sample and for the prediction of the fertilization. however there is semen samples with normal sperm morphology but with hidden sperm chromatin anomalies. since sperm morphology and chromatin anomalies reduce the fertilization rat independently, the purpose of this study is to consider the relation...

background: intrauterine insemination (iui) is one of the therapeutic approaches for infertility. the objective of this study was to evaluate dna integrity and apoptosis role in success of iui in both mild male and female factor infertility. methods: patients were divided into two groups: m (mild male factor; n=29) and f (female factor; n=31) undergoing single iui. ejaculates were analyzed and ...

Objective(s): Nicotine as a toxic substance leads to impairment of the reproductive system function. The aim of this study was to evaluate effects of melatonin on testicular alterations, sperm nuclear integrity, and epididymal sperm parameters in mice treated with nicotine.Materials and Methods: Male mice were divided into four groups. Group A received the vehicle, group B received nicotine 0.1...

Background: It is showed that methamphetamine has harmful effects on male reproductive system. The aim of this study was to investigate the effects of methamphetamine administration on testis weight, sperm count, sperm morphology, and chromatin status as well as sperm apoptotis followed by the cytochemical tests in a mouse model. Materials and Methods: Twenty four NMRI male mice were divided in...

background: isolating sperms with normal chromatin content is considered vital in intra cytoplasmic sperm injection treatment. today, researchers are trying to find new procedures to select sperms with normal chromatin content and morphology. this study examines the effectiveness of zeta, density gradient centrifugation (pure sperm) and combination of pure- zeta procedures in selecting sperms w...

Background: Sperm chromatin integrity assessment has been suggested as fertility predictor. The aim of this study was to examine the relationship between the results of sperm chromatin structure assay (SCSA) and the outcome of intrauterine insemination IUI. Materials and Methods: A total of 576 consecutive couples undergoing IUI were included and IUI was performed. SCSA results were expressed a...

in human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. the sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. the objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...

Spermatozoa are more efficient at supporting normal embryonic development than spermatids, their immature, immediate precursors. This suggests that the sperm acquires the ability to support embryonic development during spermiogenesis (spermatid to sperm maturation). Here, using Xenopus laevis as a model organism, we performed 2-D Fluorescence Difference Gel Electrophoresis (2D-DIGE) and mass sp...