BACKGROUND Reducing the percentage of sperm anomalies in insemination samples remains a goal to be achieved in the intra-cytoplasmic sperm insemination (ICSI) procedure. The aim of this study was to evaluate the efficiency of density gradient centrifugation (DGC) and Zeta methods to recover sperm with intact chromatin, and to evaluate whether the combined DGC/Zeta procedure improved ICSI outcom...

background: the aim of present study is to evaluate the effect of polyvinyl pyrrolidone (pvp) routinly used during icsi procedure on sperm membrane integrity, and sperm chromatin status. materials and methods: this study was carried out on 21 semen samples from the infertile men referring to isfahan fertility and infertility center. the processed semen samples were divided into two portions. on...

varicocele is one of the most common causes of primary male infertility. pilea microphylla (pm) is being used as folk medicine. this study was aimed to investigate the effects of pm in a rat model of varicocele. a total of 30 male wistar rats were divided into control, sham, varicocele, accessory varicocele and pm-treated groups. after 10 weeks of varicocele induction, sperm parameters and chro...

Background: The aim of this study was to examine the possible relationship between sperm DNA integrity and chromatin packaging evaluated by cytochemical assays, traditional sperm parameters and recurrent spontaneous abortion (RSA) of unknown origin. Materials and Methods: In this cohort study, 40 couples with a history of RSA and 40 couples with proven fertility were considered as case and cont...

In mouse and man Y chromosome deletions are frequently associated with spermatogenic defects. Mice with extensive deletions of non-pairing Y chromosome long arm (NPYq) are infertile and produce sperm with grossly misshapen heads, abnormal chromatin packaging and DNA damage. The NPYq-encoded multi-copy gene Sly controls the expression of sex chromosome genes after meiosis and Sly deficiency resu...

Background: Acrylamid (AA) is found in carbohydraterich foods that are prepared at high temperatures, such as French fries and potato chips. The toxic effects of AA in male animals include degeneration of epithelial cells of seminiferous tubules, decreased number of sperm and elevation in abnormal spermatozoa. The aim of this study was to evaluate the detrimental effects of AA on sperm paramete...

gibberellic acid (ga3) is a group of plant hormones identified in various plants. the aim of this study was to determine the effects of ga3 on sperm parameters and in vitro fertilization (ivf). fifty six adult male rats were divided into seven groups as, control, treatment and sham. following 15, 30 and 45 days of ga3 and methanol alcohol (ma) administration, rats were euthanized and epididymis...

introduction: the novel development of the (hba) sperm- hyaluronan binding assay has now been routinely used in some laboratories worldwide to predict sperm maturity and functionality. the purpose of this study was to evaluate the correlation between hba score and histone deficiency, protamine content and dna fragmentation. methods: during april- march 2012 patients who were enrolled at shiraz-...

background: it has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. these methods also reduce sperm chromatin deficiencies and screen the sperms before applying in assisted reproduction techniques. objective: this study compared simple density gradient methods and a combin...

background: the particles in the range of 1-100 nm are called nanoparticles56t. gold nanoparticle is one of the most important metal nanoparticles with wide usage56t.objective: 56tthis study investigated the effects of gold nanoparticles on sperm parameters and chromatin structure in mice56t.materials and methods: 56tin this experimental study, 72 male bulb-c mice were divided into 9 groups inc...