Sperm progressive motility has been reported to be one of the key factors influencing in vitro fertilization rates. However, recent studies have shown that sperm DNA fragmentation is a more robust predictor of assisted reproductive outcomes including reduced fertilization rates, embryo quality, and pregnancy rates. This study aimed to compare the usefulness of sperm progressive motility and DNA...

OBJECTIVE To examine sperm DNA fragmentation in semen used for assisted reproduction procedures to establish this factor's prognostic role in fertilization rate, embryo development, pregnancy rate, and outcome. DESIGN Prospective study. SETTING Department of Medicine and Biology of Reproduction of the Edouard Herriot Hospital in Lyon, France. PATIENT(S) 322 couples, divided into 88 cycles...

OBJECTIVE To evaluate sperm DNA fragmentation and semen parameters to diagnose male factor infertility and predict pregnancy after IVF. DESIGN Prospective study. SETTING Academic research laboratory. PATIENT(S) Seventy-five couples undergoing IVF and 28 fertile donors. INTERVENTION(S) Sperm DNA fragmentation was measured by the alkaline Comet assay in semen and sperm after density gradi...

OBJECTIVE To examine sperm motility, total antioxidant level (TAC), DNA fragmentation, and medical history in infertile men with high seminal high reactive oxygen species (ROS). DESIGN Prospective study. SETTING(S) Tertiary care hospital. PATIENT(S) Infertile men (n=101). INTERVENTION(S) Group I (n=57) included men with seminal ROS (<250 relative light units/sec/×10(6) sperm) while grou...

The aim of this report has been evaluating protamine content and DNA integrity of two patients with globozoospermia undergoing Intra Cytoplasmic Sperm Injection (ICSI). Semen analysis was carried out according to WHO criteria. Protamine deficiency and DNA fragmentation was assessed using Chromomycin A3 and sperm chromatin dispersion assay respectively. ICSI and chemical activation were carried ...

The integrity of sperm deoxyribose nucleic acid (DNA) is one of the determinants that ensure normal fertilization, implantation, pregnancy, and the generation of a healthy progeny. Assessment of sperm DNA fragmentation has gained importance as a tool to provide significant information regarding sperm quality, and it can independently predict sperm fertilizing potential. The sperm chromatin disp...

The objective of the presented study was determination of the effect of selected agents on sperm DNA fragmentation--superoxide dismutase in seminal plasma, the patients' age, and burdening with the tobacco smoking habit. An attempt was also undertaken to evaluate the effect of DNA fragmentation on the effectiveness of infertility treatment. The study covered 186 men who received treatment due t...