In this study, quantitive characteristics and chemical composition of in roach (Rutilus rutilus caspicus) sperm were investigated. Sperm traits included sperm movement duration, percentage of motile spermatozoa, sperm density, spermatocrit and sperm volume. Some seminal plasma characteristics (sodium, potassium, calcium, magnesium and chloride) were investigated. In addition, some metabolites o...

Background: Alcohol abuse is considered as one of the problems associated with poor semen production and sperm quality. Both acute and chronic alcohol consumption may affect spermatozoal chromatin disorders through apoptosis. Therefore, for the first time, this experimental study was performed to evaluate the effect of ethanol consumption on sperm parameters and chromatin integrity of spermatoz...

In this study, quantitive characteristics and chemical composition of in roach (Rutilus rutilus caspicus) sperm were investigated. Sperm traits included sperm movement duration, percentage of motile spermatozoa, sperm density, spermatocrit and sperm volume. Some seminal plasma characteristics (sodium, potassium, calcium, magnesium and chloride) were investigated. In addition, some metabolites o...

The aim of the study was to evaluate the effect of semen storage on ferrous ion induced luminescence of boar spermatozoa and to determine the relationship between parameters of this luminescence and lipid peroxidation as measured by malondialdehyde (MDA) contents. Boar semen samples were diluted in Biosolwens extender and stored for 12 days at 15 degrees C. Luminescence and MDA were measured di...

This study investigated the effects of long-term (24h) in-vitro sperm incubation at room temperature (RT; 23°C) versus testis temperature (35°C) on various sperm-quality parameters. Semen samples (n=41) were prepared both by density-gradient centrifugation (DGC) and the swim-up technique in order to compare the influence of sperm preparation on sperm quality after incubation. Progressive motili...

Background: Plasma membrane of the spermatozoa interacts with and is altered by abundant seminal PDC-109 present in their immediate milieu in vivo with damaging effect in a time- and concentration- dependent manner. Therefore we hypothesized that sequestration of detrimental protein from ejaculates would be beneficial for cryopreservation of sperm cells. To this aim we evaluated the effect of s...

Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm sample...

Heat stress has long been recognised as a cause of subfertility in farm animals. The objectives of the present study were to elucidate the effect of heat stress on sperm function and involvement of the mitogen-activated protein kinase (MAPK) 14 signalling pathway. Spermatozoa incubated for 4 h at a physiological temperature (38.5°C) exhibited significantly (P<0.05) reduced motility, plasma memb...

The aim of this study was to evaluate the effects of rapid cooling prior to freezing on frozen-thawed canine sperm quality. In experiment 1, centrifuged ejaculates from 6 dogs were pooled, split into 4 aliquots and cryopreserved by the Uppsala procedure using different cooling rates (control, cooling speed 18 C/90 min and average cooling rate 0.2 C/min; rapid, cooling speed 18 C/8 min and avera...

Male factor infertility affects 30--40% of the subfertile couples worldwide and that poses a significant medical social issue. Different strategies are developed to increase fertilization potential male gametes during procedures assisted reproduction. One these approaches is addition biologically active molecules, which help stimulate motility spermatozoa. In current investigation, we analysed ...