Spheroid cell culture emerges as powerful in vitro tool for experimental tumour research. In this study, we established a scaffold-free three-dimensional spheroid system built from canine osteosarcoma (OS) cells (D17). Spheroids (7, 14 and 19 days of cultivation) and monolayer cultures (2 and 7 days of cultivation) were evaluated and compared on light and electron microscopy. Monolayer and sphe...

Rat1-T1 and MR1 spheroids represent separate transformed phenotypes originated from the same rat fibroblasts that differ in three-dimensional (3D) growth kinetics, histological structure, and oxygenation status. In the present study, (31)P-NMR spectroscopy of perfused spheroid suspensions was used to investigate cellular energetics relative to 3D growth, development of necrosis, and cell cycle ...

The observed relation between central black hole mass and spheroid velocity dispersion is interpreted in terms of a self-regulation model that incorporates a viscous Keplerian accretion disk to feed the black hole, embedded in a massive, self-gravitating star forming disk that eventually populates the spheroid. The model leads to a constant ratio between black hole mass and spheroid mass which ...

UNLABELLED It has been long recognized that the primary advantage of imaging the brain with a positron emission tomography using GSO scintillation detectors placed on a spheroid surface is the large solid angle of acceptance for annihilation radiation, which results in improved system sensitivity and image signal-to-noise ratio. In the present study, we investigated spheroid system geometry, de...

OBJECTIVES To evaluate spheroid models of vestibular schwannoma (VS) size for bidirectional conversion of maximum diameter in the cistern and 3-dimensional volume. STUDY DESIGN Methodological study. SETTING Academic tertiary referral center. SUBJECTS AND METHODS Magnetic resonance imaging studies from 91 patients with VS from 2003 to 2011 were analyzed. Linear measurements defining meatal...

The cancer stem cell theory hypothesizes that cancer stem cells (CSCs), which possess self-renewal and other stem cell properties, are regarded as the cause of tumor formation, recurrence and metastasis. The isolation and identification of CSCs could help to develop novel therapeutic strategies specifically targeting CSCs. In this study, we enrich...

Three dimensional multicellular aggregate, also referred to as cell spheroid or microtissue, is an indispensable tool for in vitro evaluating antitumor activity and drug efficacy. Compared with classical cellular monolayer, multicellular tumor spheroid (MCTS) offers a more rational platform to predict in vivo drug efficacy and toxicity. Nevertheless, traditional processing methods such as plast...

Cells with sphere forming capacity, spheroid cells, are present in the malignant ascites of patients with epithelial ovarian cancer (EOC) and represent a significant impediment to efficacious treatment due to their putative role in progression, metastasis and chemotherapy resistance. The exact mechanisms that underlie EOC metastasis and drug resistance are not clear. Understanding the biology o...

As part of a global analysis of deep star counts to constrain scenarii of galaxy formation and evolution, we investigate possible links between the galactic spheroid and the dark matter halo. A wide set of deep star counts at high and intermediate galactic latitudes is used to determine the large scale density law of the spheroid. Assuming a power density law, the exponent, flattening, local de...

Colon cancer stem cells (CCSCs) are involved in colon cancer and promote tumor progression and recurrence. LGR5, a marker for intestinal stem cells (ISCs), is also considered to serve as a marker for CCSCs. However, the precise function of LGR5 in CCSCs is unclear. In this study, we demonstrated that LGR5 was highly expressed in CCSCs-enriched HT29 spheroid cells. Downregulation of LGR5 with sm...