OBJECTIVE Thoracentesis is the first investigation to be performed in a patient with lung cancer and pleural effusion. The diagnostic yield of conventional smear studies varies in the first thoracentesis. In this study, we aimed to investigate if the cell block method increases the diagnostic yield in exudative pleural effusions accompanying lung cancer. MATERIAL AND METHOD Forty patients wit...

Peritoneal macrophages of mice, 48 hr after intraperitoneal administration of albumin (egg white), were studied. Almost all granules in the cytoplasm of macrophages stained by the supravital staining with neutral red were revealed to be colored brick red by the staining with Sudan III in the smear preparation. Because of lack of lipids in the inoculum, it is difficult to explain that these gran...

We studied 27 patients diagnosed with pericardial effusion with cardiac tamponade on which pericardiocentesis was performed. The purpose of the study was to evaluate the benefits and limits of the cytological examination of the pericardial liquid in the etiological diagnosis and the treatment of patients with cardiac tamponade. The pericardial liquid taken was examined macroscopically, biochemi...

BACKGROUND & OBJECTIVES Making centrifuged deposit smears from sputum to detect acid-fast bacilli (AFB) is considered hazardous. We carried out this study to stain the centrifuged deposits with carbol-fuchsin in sputum containers and to decolourize and counterstain their smears made on glass slides. METHODS The centrifuged deposits of 180 sputum samples from pulmonary tuberculosis patients we...

Objective behind the study was to assess the efficacy of Toluidine Blue (TB) mouth rinse in the detection of neoplasia in oral cavity and to evaluate its usefulness in high risk groups. Correlation of the results of scrape cytology smear and histopathology of biopsy taken from the TB stained area was also done. This prospective study was carried out on 85 cases of suspected oral lesions. Comple...

Material and Methods A series of 100 patients with trachoma in the infiltrative or follicular stage, in which inclusion bodies could be seen, was selected for this study. Conjunctival scrapings were obtained from the upper fornix by a sharp curette and spread on a slide, care being taken that no blood oozed out. The scrapings were stained by methylene blue and citric acid stain according to the...

Background Tuberculosis (TB) is a common and lethal infectious disease, which requires accurate and early diagnosis for effective containment. Essential for the diagnosis of pulmonary infection is the detection of the bacilli through the manual microscopic examination of ZN-stained sputum smear, which is a time-consuming, complex process necessitating at least 8-10 minutes per slide. Moreover, ...

Sputum smear observation has an important role in tuberculosis (TB) disease diagnosis, because it needs accurate identification to avoid high errors diagnosis. In development countries, sputum smear slide observation is commonly done with conventional light microscope from Ziehl-Neelsen stained tissue and it doesn’t need high cost to maintain the microscope. The clinicians do manual screening p...

Objective: to know and document the risk of factors for category leishmaniasis. Material and Methods: Cutaneous leishmaniasis is rapidly and easily diagnosed by direct examination of impression smear from lesion. Smear was stained by giemsa or Wright stain. In active lesion amastigote form of parasite are easily recognized intracellularly and extracellularly. Diagnosis of visceral leishmaniasis...

A polymerase chain reaction (PCR) assay for the rapid detection of Mycobacterium tuberculosis in sputum samples was studied. The target DNAwas a 123-base pair (bp) fragment ofIS6110, which was repeated in the M.tuberculosis genomeand was specific for the M.tuberculosis complex. Glass beads (2mmdiameter) and lysozyme were used to lyse the mycobacteria and DNAwas extracted by the phenol-extractio...