Flow cytometry was utilized to measure the influx of calcium into fresh and frozen-thawed equine and bovine spermatozoa that had been treated with either dilauroyl-phosphatidylcholine ( PC-12) or the calcium ionophore A23187 (A23187) to initiate sperm capacitation. Sperm suspensions were stained with the fluorescent calcium indicator, Fluo-3, to measure intracellular calcium concentrations and ...

Mitochondria have been proposed as the major source of reactive oxygen species in somatic cells and human spermatozoa. However, no data regarding the role of mitochondrial ROS production in stallion spermatozoa are available. To shed light on the role of the mitochondrial electron transport chain in the origin of oxidative stress in stallion spermatozoa, specific inhibitors of complex I (roteno...

The aims of this study were to compare glycerol (G) at customary concentrations and ethylene glycol (EG) as cryoprotectants for stallion semen in a skimmed milk (SM) extender, to test different EG concentrations and to compare the results of manual and computerized analysis with the hypoosmotic swelling (HOS) test. Ejaculates from two stallions were collected over 3 weeks (6 ejaculates per stal...

Descriptions of reproductive behavior of equids under free-running conditions are available in a number of resources.* These can be very instructive toward understanding the basic nature of stallions and how behavior varies under domestic breeding conditions. Most domestic stallions are handled under halter for live breeding or collection of semen. As opposed to the full complement of harem for...

a seven-year-old stallion with diversion of urinary pass to the thigh due to a urethral fistula caused by gunshot perineal injury was presented to the urmia university, veterinary teaching hospital. no signs of urinary obstruction or renal failure were observed. because of inaccessible location of the injured urethra, through a perineal urethrotomy incision, a urinary catheter was effectively a...

Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytom...

A 13-year-old Tennessee Walking Horse stallion developed acute onset of colic in central Georgia in October. He was found in his stall in the morning with numerous abrasions on his head. The grain and hay that he was offered the previous evening were only partially consumed. The owner witnessed him pawing at the ground. He then became recumbent and rolled. The local veterinarian was called and ...

Study objectives were to; 1) determine if supplementing of n-3 fatty acids improves membrane integrity, and hence viability and motility of stallion spermatozoa following cold storage, and following cryopreservation, and 2) determine if n-3 supplementation alters the fatty acid composition of stallion spermatozoa. Data indicate that following 90 d of n-3 supplementation daily sperm output and t...

stallions diluted at a ratio of 3:1 (extender: semen) with a commercially available stallion semen extender (Exodus Breeders Supply, York, PA) and packaged into each of the containers. Three sets of containers per season (winter and summer) where commercially shipped from Tucson, AZ to Springfield, MO with overnight delivery. Spermatozoal motilities were evaluated utilizing CASA following shipm...

Spermatogonial stem cell transfer has been studied in various species and suggested potential for success. Bull, stallion, donkey, mule, boar, dog and cat testicles were examined using various injection techniques to determine distribution patterns of injected material most favorable for stem cell transfer. Spermatogonial stem cells were recovered from freshly harvested stallion testicles using...