bacterial resistance to antibiotics is a serious problem and is increasing in prevalence world-wide at an alarming rate. the antimicrobial susceptibility patterns of 1897 gram-positive bacterial isolates were evaluated. the minimum inhibitory concentration (mic) of isolates which comprised staphylococcus aureus (927 isolates), coagulase-negative staphylococci (cns; 425 isolates), enterococcus f...

Nosocomial infection caused by methicillin-resistant staphylococci poses a serious problem in many countries. The aim of this study was to rapidly and reliably detect methicillin-resistant-staphylococci in order to suggest appropriate therapy. The presence or absence of the methicillin-resistance gene in 115 clinical isolates of Staphylococcus aureus and 50 isolates of Coagulase Negative Staphy...

During 1984, 35 patients undergoing continuous peritoneal dialysis experienced 77 cases of peritonitis with 55 cases (71.4%) related to staphylococci. Coagulase-negative staphylococci were isolated in 41 cases, while Staphylococcus aureus was found in 14. A coexisting tunnel infection was more often associated with S. aureus (7/14) than with coagulase-negative staphylococci (2/41) (P less than ...

OBJECTIVE To determine the prevalence of oral staphylococcal carriage in patients with rheumatoid arthritis compared with healthy controls. METHODS Fifty healthy adults, 25 healthy elderly volunteers and 25 patients with rheumatoid arthritis were studied. An oral rinse, tongue swab and nasal swab were collected for culture on blood agar and a range of selective agars. Isolates of staphylococc...

results of total 60 s. aureus isolates, 51 (85%) were mrsa, and of 60 cons, 7 (11.66%) were mrcons. the results showed that the mic of chlorhexidine for all 120 isolates was 1-16 μg/ml. 15 (12.5%) isolates carried qaca/b gene, 26 (21.7%) carried qacc gene, and 38 (31.7%) carried smr gene. conclusions maintenance of mrsa isolates in the attendance of low amounts of antiseptics could result in th...

Four commercially available rapid agglutination tests for the identification of Staphylococcus aureus were compared with the tube coagulase test for the identification of 300 methicillin-resistant isolates of staphylococci. Isolates tested included 207 methicillin-resistant S. aureus and 93 coagulase-negative staphylococci, collected from five medical centers. Strain variability was documented ...

The antibiotic susceptibility test was carried out on 103 Staphylococcus aureus and 136 coagulase-negative staphylococci (CNS) strains. Only 35 of the isolates were susceptible to all antibiotics tested, while the remaining 204 isolates were resistant at least to one of the antibiotics. Among staphylococci, 18 isolates of S. aureus and 31 isolates of CNS were found phenotypically resistant to m...

Anchoring of Staphylococcus aureus surface protein to the cell wall is catalyzed by sortase, a transpeptidase. The contribution of staphylococcal surface proteins to establishment of infection was examined using a murine septic arthritis model. Intravenous inoculation of mice with the sortase-deficient mutant S. aureus strain SMK3 did not result in weight loss or severe septic arthritis, in con...