A total of 353 coagulase-negative staphylococcus (CNS) isolates from infected bovine mammary glands were tested for cytolysin production by using the synergistic hemolysin assay (SHA). Overall, 34.6% of CNS isolates were SHA positive. Human-associated, coagulase-negative staphylococcal species contained the greatest number of SHA-positive strains. Milk leukocyte levels expressed as somatic cell...

We read with interest the paper by Layer et al. regarding the identification of staphylococci with automated systems including VITEK-2 (bioMérieux, Marcy l’Etoile, France) (6). Of 86 strains analyzed in their study, 4 were Staphylococcus hominis and all were correctly identified by the colorimetric ID-GP VITEK-2 cards. Identical results have been reported in recent studies evaluating 13 and 14 ...

We describe a new, efficient, sensitive, and fast single-tube multiple-PCR protocol for the identification of the most clinically significant Staphylococcus spp. and the simultaneous detection of the methicillin and mupirocin resistance loci. The protocol identifies at the species level isolates belonging to S. aureus, S. epidermidis, S. haemolyticus, S. hominis, S. lugdunensis, and S. saprophy...

Thirteen coagulase-negative, oxidase-negative, and novobiocin-susceptible staphylococci were isolated from human clinical specimens. The isolates were differentiated from known staphylococcal species on the basis of 16S rRNA, hsp60, rpoB, dnaJ, tuf, and gap gene sequencing, automated ribotyping, (GTG)5-PCR fingerprinting, and MALDI-TOF MS analysis. Phylogenetic analysis based on the 16S rRNA ge...

A total of 221 strains of staphylococci and 98 strains of micrococci isolated from the skins of Eastern gray squirrels, Southern flying squirrels, raccoons, opossums, squirrel monkeys, swine, sheep, horses, cattle, and dogs were characterized in a preliminary attempt to resolve their natural relationships and distribution in nature. Staphylococci demonstrating the widest host range included Sta...

A collection of 99 staphylococcal isolates associated with prosthetic joint infection and 23 coagulase-negative staphylococci isolated from noninfected arthroplasty-associated specimens were screened in order to determine whether the presence of icaA could be used to distinguish between pathogens and nonpathogens. All Staphylococcus aureus prosthetic joint infection isolates (n = 55) were icaA ...

INTRODUCTION To evaluate the efficacy of Daptomycin (DPT) lock therapy in the treatment of Grampositive long-term catheter-related bloodstream infections (LT-CRBI). PATIENTS AND METHODS A retrospective review of all patients receiving DPT lock therapy for the treatment of LT-CRBI from December 2009 to May 2010 was conducted. The primary endpoint used in this study was failure to cure the epis...