Neurohormones diffuse in sweat and epidermis leading skin bacterial microflora to be largely exposed to these host factors. Bacteria can sense a multitude of neurohormones, but their role in skin homeostasis was only investigated recently. The first study focused on substance P (SP), a neuropeptide produced in abundance by skin nerve terminals. SP is without effect on the growth of Gram-positiv...

In the present study, aqueous and solvent extracts of leaves collected from nine mangrove plants were screened against Methicillin resistant Staphylococcus aureus (MRSA), Vancomycin resistant Staphylococcus aureus (VRSA) and Extended spectrum beta lactamase (ESBL) strains. Out of nine mangrove plants Avicennia sp., Rhizophora sp., Ceriops decandra and Thillai species showed antimicrobial activi...

Crude extracts were prepared using the solvent ethanol from the seaweeds viz., Gracilaria edulis, Calorpha peltada and Hydroclothres sp. and screening for their antibacterial activity against six bacterial pathogens were also carried out. The test bacterial strains were Escherichia coli, Enterobacter aerogenes, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus faecalis and Bacillus c...

The present study aimed evaluate an on-farm culture system for identification of milk pathogens associated with clinical mastitis in dairy cows using two different gold standard approaches: standard laboratory culture in study 1 and 16S rRNA sequencing in study 2. In study 1, milk from mastitic quarters (i.e. presence of flakes, clots, or serous milk; n = 538) was cultured on-farm using a singl...

Some strains of Staphylococcus epidermidis and Micrococcus sp. produce nucleases. However, thermal stability was shown to be unique to the nucleases of S. aureus. In addition, two micromethods for susceptibility testing to lysostaphin were more precise and convenient than anaerobic glucose fermentation in distinguishing between the genera Staphylococcus and Micrococcus.

The objective of this study was to investigate the antibacterial activity of primary and secondary metabolites from Vibrio alginoliticus isolated from sponge Haliclona sp. against Staphylococcus aureus. A descriptive method was used in this research. The antibacterial activity was analysed by paper disk method. The results showed that the primary metabolites produced by Vibrio alginoliticus tha...

A 12-year-old child developed meningitis 6 days after a third ventriculostomy by endoscopy. A coagulase-negative Staphylococcus sp. was isolated in pure culture from the cerebrospinal fluid and was definitely identified as Staphylococcus lugdunensis after the 16S ribosomal DNA gene and rpoB gene were sequenced. This report describes the first case of S. lugdunensis meningitis.

The microbial composition of smear-ripened cheeses is not very clear. A total of 194 bacterial isolates and 187 yeast isolates from the surfaces of four Irish farmhouse smear-ripened cheeses were identified at the midpoint of ripening using pulsed-field gel electrophoresis (PFGE), repetitive sequence-based PCR, and 16S rRNA gene sequencing for identifying and typing the bacteria and Fourier tra...

conclusions the streptomyces sp. suk 25 culturing was optimized using thronton media, at ph 7 and aeration of 140 rpm. further isolation and identification of bioactive compounds will develop anti-mrsa therapeutics. materials and methods disc diffusion and minimum inhibitory concentration (mic) assay were used to determine the antibacterial activity of streptomyces sp. isolates. optimization of...

An organic solvent tolerant lipase gene from Staphylococcus epidermidis AT2 was successfully cloned and expressed with pTrcHis2 in E. coli TOP10. Sequence analysis revealed an open reading frame (ORF) of 1,933 bp in length which coded for a polypeptide of 643 amino acid residues. The polypeptide comprised of a signal peptide (37 amino acids), pro-peptide and a mature protein of 390 amino acids....