This paper describes protein expression inside highly stable and oil-free giant vesicles (GVs) that are prepared by deforming a planar lipid membrane using a pulsed jet flow (Fig. 1). We found that the encapsulation efficiency of plasmid DNA into GVs formed by the pulsed jet flow was 7 times higher than that by conventional methods (e.g. gentle hydration method). Moreover, green fluorescent pro...

CMOS is used to construct the integrated circuits with low level of static leakage. With this low level leakage we are designing all the transistor circuits in CMOS logic. To control this static leakage in the circuits the supply voltage is a major concern. Here the step-up converters with charge pump and the level for maintaining its threshold voltage (VT) is to be analyzed and proposed. Here ...

we introduce a new one-step iteration process to approximate common fixed points of twononexpansive mappings in banach spaces and prove weak convergence of the iterative sequence using (i)opial’s condition and (ii) kadec-klee property. strong convergence theorems are also established in banachspaces and uniformly convex banach spaces under the so-called condition ( a ), which is weaker thancom...

Electrostatic potential as well as the local volume charge density are computed for a macromolecule by solving the Poisson-Boltzmann equation (PBE) using the finite element method (FEM). As a verification, our numerical results for a one dimensional PBE, which corresponds to an infinite-length macromolecule, are compared with the existing analytical solution and good agreement is found. As a ma...

The fixed charge (fixed cost) may present the cost of renting a vehicle, landing fees in an airport, setup cost for machines in a manufacturing environment, etc. In this paper, we discuss fixed charge capacitated in a non-linear transportation problem. Thereby, we establish local optimum condition of this problem. Next we establish an algorithm for solving this transportation problem. Also, we ...

In this paper, we introduce a class of stochastic programming problem with fixed charge recourse in which a fixed cost is imposed if the value of the continuous recourse variable is strictly positive. The algorithm of a branch-and-cut method to solve the problem is developed by using the property of the expected recourse function. Then, the problem is applied to a power generating system. The n...

Numerous planning models within the chemical, petroleum, and process industries involve coordinating the movement of raw materials in a distribution network so that they can be blended into final products. The uncapacitated fixed-charge transportation problem with blending (FCTPwB) studied in this paper captures a core structure encountered in many of these environments. We model the FCTPwB as ...

A realistic model of a fixed-charge membrane was constructed to elucidate the principles involved in the Teorell-Meyer-Sievers theory. Polyelectrolyte solutions (polystyrene sulfonic acid or gelatin) were used in a multicompartment cell to serve as the "membrane." The ionic concentration and potential differences between the compartments served as a finite increment approximation to a continuou...

We consider a class of fixed-charge transportation problems over graphs. We show that this problem is strongly NP-hard, but solvable in pseudo-polynomial time over trees using dynamic programming. We also show that the LP formulation associated to the dynamic program can be obtained from extended formulations of single-node flow polytopes. Given these results, we present a unary expansion-based...