The pandemic of lipid-related disease necessitates a determination of how cholesterol and other lipids are transported and stored within cells. The first step in this determination is the identification of the genes involved in these transport and storage processes. Using genome-wide screens, we identified 56 yeast (Saccharomyces cerevisiae) genes involved in sterol-lipid biosynthesis, intracel...

A series of LM cell variants resistant to filipin, but not auxotrophic for sterol, was isolated by plating mutagenized, filipin-treated cells on soft agar medium containing no sterol. Cloned variants were assayed for growth in the presence and absence of sterol or unsaturated fatty acid. Filipin-resistant clones whose growth rate was unaffected by the addition of sterol to the medium were furth...

Using the terms of (Reiter and Dale, 2000), we consider that the Document Planner architecture is pipelined: first the content determination task does its work, and then the document structuring task takes the result and build a document plan. Following the work of (Roussarie, 2000), we adopt SDRT (Asher, 1993; Asher and Lascarides, 1998), which was designed first for text understanding, for th...

Synaptic vesicles have a high sterol content, but the importance of vesicular sterols during vesicle recycling is unclear. We used the Drosophila temperature-sensitive dynamin mutant, shibire-ts1, to block endocytosis of recycling synaptic vesicles and to trap them reversibly at the plasma membrane where they were accessible to sterol extraction. Depletion of sterols from trapped vesicles preve...

Hand-crafted approaches to content determination are expensive to port to new domains. Machine-learned approaches, on the other hand, tend to be limited to relatively simple selection of items from data sets. We observe that in time series domains, textual descriptions often aggregate a series of events into a compact description. We present a simple technique for automatically determining sequ...

In this paper we describe a two-stage model for content determination in systems that summarise time series data. The first stage involves building a qualitative overview of the data set, and the second involves using this overview, together with the actual data, to produce summaries of the timeseries data. This model is based on our observations of how human experts summarise time-series data.

T HE effects of oven drying temperature and drying time on whole-kernel, long-grain rough rice moisture content determination were investigated for different moisture content levels ranging from approximately 9 to 22% (w.b.). The results showed that a simplified oven method can be used for rapid moisture measurement with accuracy comparable to that of a standard Association of Official Analytic...

Genes encoding sterol ester-forming enzymes were recently identified in the Arabidopsis (Arabidopsis thaliana) genome. One belongs to a family of six members presenting homologies with the mammalian Lecithin Cholesterol Acyltransferases. The other one belongs to the superfamily of Membrane-Bound O-Acyltransferases. The physiological functions of these genes, Phospholipid Sterol Acyltransferase1...

These studies test the hypothesis that a major determinant of excessive biliary cholesterol secretion is a level of hepatic sterol synthesis that is inappropriately high relative to the needs of the liver cell for preserving cholesterol balance. Biliary cholesterol secretion was measured in vivo in two models after loading the hepatocyte with sterol by two different mechanisms. In the first mod...

Mutants of the genes nys1 and nys3 differ from sensitive strains (nys(+)) in their sterol content. Ultraviolet absorption spectra of the nonsaponifiable material extracted from cells of nys(+) demonstrated the presence of ergosterol and 24(28)-dehydroergosterol. In nys1 mutants, the spectrum suggests the presence of a new sterol. The absorption spectrum of extracts from nys3 mutants indicates a...