Nine Dictyuchus isolates were obtained from floating and decaying twigs and leaves in Anzali lagoon, Anzali County, Guilan province, Iran. They are interestingly distinguished from current Dictyuchus species by the presence of dictyoid and achlyoid type of zoospore discharge, abundant fusiform and spherical gemma, and the absence of any sexual apparatus. In addition, phylogene...

The hyper-variable V4 and V9 regions of the small subunit (SSU) rDNA have been targeted for assessing environmental diversity of microbial eukaryotes using next generation sequencing technologies. Here, we explore how the genetic distances among these short fragments compare with the distances obtained from near full-length SSU-rDNA sequences by comparing all pairwise estimates, as well as with...

This study presents the suitability of D1/D2 domain of large-subunit (LSU) ribosomal DNA (rDNA) for differentiation of Orpinomyces joyonii and Orpinomyces intercalaris based on PCR-restriction fragment length polymorphism (RFLP). A variation of G/T in O. intercalaris created an additional restriction site for AluI, which was used as an RFLP marker. The results demonstrate adequate heterogeneity...

The genus Neoconidiobolus, typified by N. thromboides (Drechsler) B. Huang & Y. Nie, was recently established to accommodate all members of the Conidiobolus subgenus which produces neither microconidia nor capilliconidia. Based on mitochondrial small subunit (mt SSU rDNA), nuclear large ribosomal (nc LSU and translation elongation factor 1-alpha (TEF1), we further resolved Neoconidiobolus into ...

Infections by ectoparasitic trichodinid ciliates cause epidermal damages to artificially reared juvenile barfin flounder Verasper moseri of mariculture fisheries in Hokkaido, northern Japan. ​In the present study, morphological observation and molecular phylogenetic analysis using small subunit ribosomal RNA gene (SSU rDNA) were performed identify ciliates. ​Morphological denticles adoral cilia...

A parasitological study carried out in May 2022 and March 2023 the Nyando River of Lake Victoria Basin, Kenya, disclosed two parasitic lernaeid copepods: Lamproglena cleopatra Humes, 1957, from gills a cyprinid, Ningu Labeo victorianus Boulenger, 1901, endemic to drainage system, clariae Fryer, clariid, North African catfish Clarias gariepinus (Burchell, 1822). The copepods were studied supplem...

Mitochondrial and nuclear DNA sequence data for 105 acanthomorph taxa are analyzed to address questions of scorpaeniform monophyly and relationships. The combination of 3425 aligned base pairs from the mitochondrial small subunit rDNA (12S), large subunit rDNA (16S), and tRNA-Val and the nuclear large subunit rDNA (28S), histone H3, and TMO-4c4 loci are analyzed. Representatives of all scorpaen...